FN Archimer Export Format PT J TI Kinetic study of solid phase demineralization by weak acids in one-step enzymatic bio-refinery of shrimp cuticles BT AF BARON, Regis SOCOL, Marius ARHALIASS, A. BRUZAC, Sandrine LE ROUX, Karine DEL PINO, J. Rodriguez BERGE, Jean-Pascal KAAS, Raymond AS 1:1;2:1;3:4;4:1;5:3;6:1;7:1;8:2; FF 1:PDG-RBE-BRM;2:PDG-RBE-BRM-BIORAPHE;3:;4:PDG-RBE-BE-LBCM;5:;6:;7:PDG-RBE-BRM-BIORAPHE;8:PDG-RBE-BRM-PBA; C1 IFREMER, Biorafhe, F-44311 Nantes 03, France. IFREMER, PBA, F-44311 Nantes 03, France. Ynsect, F-44311 Nantes 03, France. Univ Nantes, GEPEA, CNRS, UMR 6144,CRTT, F-44600 St Nazaire, France. C2 IFREMER, FRANCE IFREMER, FRANCE YNSECT, FRANCE UNIV NANTES, FRANCE SI NANTES SE PDG-RBE-BRM PDG-RBE-BRM-BIORAPHE PDG-RBE-BE-LBCM PDG-RBE-BRM-PBA IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.529 TC 11 UR https://archimer.ifremer.fr/doc/00281/39244/37809.pdf LA English DT Article DE ;Bio-refinery;Acidic enzymatic proteolysis;Shrimp cuticles;Demineralization kinetics and modeling AB We describe a one-step bio-refinery process for shrimp composites by-products. Its originality lies in a simple rapid (6 h) biotechnological cuticle fragmentation process that recovers all major compounds (chitins, peptides and minerals in particular calcium). The process consists of a controlled exogenous enzymatic proteolysis in a food-grade acidic medium allowing chitin purification (solid phase), and recovery of peptides and minerals (liquid phase). At a pH of between 3.5 and 4, protease activity is effective, and peptides are preserved. Solid phase demineralization kinetics were followed for phosphoric, hydrochloric, acetic, formic and citric acids with pKa ranging from 2.1 to 4.76. Formic acid met the initial aim of (i) 99 % of demineralization yield and (ii) 95 % deproteinization yield at a pH close to 3.5 and a molar ratio of 1.5. The proposed one-step process is proven to be efficient. To formalize the necessary elements for the future optimization of the process, two models to predict shell demineralization kinetics were studied, one based on simplified physical considerations and a second empirical one. The first model did not accurately describe the kinetics for times exceeding 30 minutes, the empirical one performed adequately. PY 2015 PD DEC SO Process Biochemistry SN 1359-5113 PU Elsevier Sci Ltd VL 50 IS 12 UT 000367125900027 BP 2215 EP 2223 DI 10.1016/j.procbio.2015.09.017 ID 39244 ER EF