FN Archimer Export Format PT J TI Calcium response of KCl-excited populations of ventricular myocytes from the European sea bass (Dicentrarchus labrax): a promising approach to integrate cell-to-cell heterogeneity in studying the cellular basis of fish cardiac performance BT AF OLLIVIER, Helene MARCHANT, James LE BAYON, Nicolas SERVILI, Arianna CLAIREAUX, Guy AS 1:1;2:2;3:2;4:2;5:2; FF 1:;2:;3:PDG-RBE-PFOM-ARN;4:PDG-RBE-PFOM-ARN;5:; C1 Univ Bretagne Occidentale, ORPHY, Brest, France. Ctr Ifremer Brest, LEMAR, Unite PFOM ARN, Plouzane, France. C2 UBO, FRANCE IFREMER, FRANCE SI BREST SE PDG-RBE-PFOM-ARN UM LEMAR IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 1.884 TC 4 UR https://archimer.ifremer.fr/doc/00283/39412/37904.pdf LA English DT Article DE ;Sea bass;Myocyte populations;Calcium signaling;Cardiac plasticity;Inter-individual variability AB Climate change challenges the capacity of fishes to thrive in their habitat. However, through phenotypic diversity, they demonstrate remarkable resilience to deteriorating conditions. In fish populations, inter-individual variation in a number of fitness-determining physiological traits, including cardiac performance, is classically observed. Information about the cellular bases of inter-individual variability in cardiac performance is scarce including the possible contribution of excitation-contraction (EC) coupling. This study aimed at providing insight into EC coupling-related Ca2+ response and thermal plasticity in the European sea bass (Dicentrarchus labrax). A cell population approach was used to lay the methodological basis for identifying the cellular determinants of cardiac performance. Fish were acclimated at 12 and 22 A degrees C and changes in intracellular calcium concentration ([Ca2+](i)) following KCl stimulation were measured using Fura-2, at 12 or 22 A degrees C-test. The increase in [Ca2+](i) resulted primarily from extracellular Ca2+ entry but sarcoplasmic reticulum stores were also shown to be involved. As previously reported in sea bass, a modest effect of adrenaline was observed. Moreover, although the response appeared relatively insensitive to an acute temperature change, a difference in Ca2+ response was observed between 12- and 22 A degrees C-acclimated fish. In particular, a greater increase in [Ca2+](i) at a high level of adrenaline was observed in 22 A degrees C-acclimated fish that may be related to an improved efficiency of adrenaline under these conditions. In conclusion, this method allows a rapid screening of cellular characteristics. It represents a promising tool to identify the cellular determinants of inter-individual variability in fishes' capacity for environmental adaptation. PY 2015 PD OCT SO Journal Of Comparative Physiology B-Biochemical Systemic And Environmental Physiology SN 0174-1578 PU Springer Heidelberg VL 185 IS 7 UT 000361471600004 BP 755 EP 765 DI 10.1007/s00360-015-0924-6 ID 39412 ER EF