FN Archimer Export Format
PT J
TI Effect of temperature, food availability, and estradiol injection on gametogenesis and gender in the pearl oyster     Pinctada margaritifera
BT 
AF TEANINIURAITEMOANA, Vaihiti
   LEPRETRE, Maxime
   LEVY, Peva
   VANAA, Vincent
   PARRAD, Sophie
   GAERTNER-MAZOUNI, Nabila
   GUEGUEN, Yannick
   HUVET, Arnaud
   LE MOULLAC, Gilles
AS 1:1;2:1;3:1;4:1;5:1;6:2;7:3;8:4;9:1;
FF 1:PDG-RBE-RMPF;2:;3:PDG-RBE-RMPF;4:PDG-RBE-RMPF;5:PDG-RBE-HMMN-RHPEB;6:;7:PDG-RBE-IHPE;8:PDG-RBE-PFOM-PI;9:PDG-RBE-RMPF;
C1 IFREMER, UMR Ecosyst Insulaires Oceaniens EIO 241, Labex CORAIL, Ctr Pacifique, Taravao, Tahiti, Fr Polynesia.
   Univ Polynesie Francaise, UMR Ecosyst Insulaires Oceaniens EIO 241, Labex CORAIL, Faaa, Tahiti, Fr Polynesia.
   Univ Montpellier, IFREMER, UMR Interact Hotes Pathogenes Environm IHPE 5244, F-34059 Montpellier 5, France.
   IFREMER, ZI Pointe du Diable, UMR Lab Sci Environm Marin LEMAR 6539, Plouzane, France.
C2 IFREMER, FRANCE
   UNIV POLYNESIE FRANCAISE, FRANCE
   UNIV MONTPELLIER, FRANCE
   IFREMER, FRANCE
SI TAHITI
   PORT-EN-BESSIN
   MONTPELLIER
   BREST
SE PDG-RBE-RMPF
   PDG-RBE-HMMN-RHPEB
   PDG-RBE-IHPE
   PDG-RBE-PFOM-PI
UM LEMAR
   IHPE
   EIO
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.28
TC 23
UR https://archimer.ifremer.fr/doc/00285/39638/38184.pdf
LA English
DT Article
AB The black-lip pearl oyster Pinctada margaritifera is a protandrous hermaphrodite species. Its economic value has led to the development of controlled hatchery reproduction techniques, although many aspects remain to be optimized. In order to understand reproductive mechanisms and their controlling factors, two independent experiments were designed to test hypotheses of gametogenesis and sex ratio control by environmental and hormonal factors. In one, pearl oysters were exposed under controlled conditions at different combinations of temperature (24 and 28°C) and food level (10,000 and 40,000 cells mL−1); whereas in the other, pearl oysters were conditioned under natural conditions into the lagoon and subjected to successive 17β-estradiol injections (100 μg per injection). Gametogenesis and sex ratio were assessed by histology for each treatment. In parallel, mRNA expressions of nine marker genes of the sexual pathway (pmarg-foxl2, pmarg-c43476, pmarg-c45042, pmarg-c19309, pmarg-c54338, pmarg-vit6, pmarg-zglp1, pmarg-dmrt, and pmarg-fem1-like) were investigated. Maximum maturation was observed in the treatment combining the highest temperature (28°C) and the highest microalgae concentration (40,000 cells mL−1), where the female sex tended to be maintained. Injection of 17β-estradiol induced a significant increase of undetermined stage proportion 2 weeks after the final injection. These results suggest that gametogenesis and gender in adult pearl oysters can be controlled by environmental factors and estrogens. While there were no significant effects on relative gene expression, the 3-gene-pair expression ratio model of the sexual pathway of P. margaritifera, suggest a probable dominance of genetic sex determinism without excluding a mixed sex determination mode (genetic + environmental)
PY 2016
PD JAN
SO Journal Of Experimental Zoology Part A-ecological Genetics And Physiology
SN 1932-5223
PU Wiley-blackwell
VL 325
IS 1
UT 000368727400002
BP 13
EP 24
DI 10.1002/jez.1992
ID 39638
ER

EF