FN Archimer Export Format
PT J
TI Rapid detection and quantification of the marine toxic algae, Alexandrium minutum, using a super-paramagnetic immunochromatographic strip test
BT 
AF GAS, Fabienne
   BAUS, Beatrice
   QUERE, Julien
   CHAPELLE, Annie
   DREANNO, Catherine
AS 1:1;2:1;3:2;4:2;5:3;
FF 1:;2:;3:PDG-ODE-DYNECO-PELAGOS;4:PDG-ODE-DYNECO-PELAGOS;5:PDG-REM-RDT-LDCM;
C1 CEA Marcoule, Direct Sci Vivant, Lab Innovat Technol Detect & Diagnost, F-30200 Bagnols Sur Ceze, France.
   IFREMER, Ctr Brest, Dyneco Pelagos, F-29280 Plouzane, France.
   IFREMER, Ctr Brest, Lab Detect Capteurs & Mesures, F-29280 Plouzane, France.
C2 CEA, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
   PDG-REM-RDT-LDCM
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 4.162
TC 21
UR https://archimer.ifremer.fr/doc/00286/39695/38152.pdf
LA English
DT Article
DE ;Alexandrium minutum;Detection;Quantification;Dipstick lateral flow immunoassay;Super-paramagnetic
AB The dinoflagellates of Alexandrium genus are known to be producers of paralytic shellfish toxins that regularly impact the shellfish aquaculture industry and fisheries. Accurate detection of Alexandrium including A. minutum is crucial for environmental monitoring and sanitary issues. In this study, we firstly developed a quantitative lateral flow immunoassay (LFIA) using super-paramagnetic nanobeads for A. minutum whole cells. This dipstick assay relies on two distinct monoclonal antibodies used in a sandwich format and directed against surface antigens of this organism. No sample preparation is required. Either frozen or live cells can be detected and quantified. The specificity and sensitivity are assessed by using phytoplankton culture and field samples spiked with a known amount of cultured A. minutum cells. This LFIA is shown to be highly specific for A. minutum and able to detect reproducibly 105 cells/L within 30 min. The test is applied to environmental samples already characterized by light microscopy counting. No significant difference is observed between the cell densities obtained by these two methods. This handy super-paramagnetic lateral flow immnunoassay biosensor can greatly assist water quality monitoring programs as well as ecological research.
PY 2016
PD JAN
SO Talanta
SN 0039-9140
PU Elsevier Science Bv
VL 147
UT 000366078200081
BP 581
EP 589
DI 10.1016/j.talanta.2015.10.036
ID 39695
ER

EF