FN Archimer Export Format
PT J
TI Genetic diversity analysis of isolates belonging to the Photobacterium phosphoreum species group collected from salmon products using AFLP fingerprinting
BT 
AF JEROME, Marc
   MACE, Sabrina
   DOUSSET, Xavier
   POT, Bruno
   JOFFRAUD, Jean-Jacques
AS 1:1;2:3;3:2;4:4;5:1;
FF 1:PDG-RBE-BRM-LEMMMB;2:;3:;4:;5:PDG-RBE-BRM-LEMMMB;
C1 IFREMER, Lab Ecosyst Microbiens & Mol Marines Biotechnol E, F-44311 Nantes, France.
   LUNAM Univ, UMR1014, Nantes, France.
   Univ Liege, Dept Food Sci, Fundamental & Appl Res Anim & Hlth, Food Microbiol, B-4000 Liege, Belgium.
   Appl Maths NV, Sint Martens Latem, Belgium.
C2 IFREMER, FRANCE
   UNIV NANTES, FRANCE
   UNIV LIEGE, BELGIUM
   APPL MATHS NV, BELGIUM
SI NANTES
SE PDG-RBE-BRM-LEMMMB
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 3.339
TC 10
UR https://archimer.ifremer.fr/doc/00286/39712/38167.pdf
LA English
DT Article
DE ;Photobacterium phosphoreum;AFLP;gyrB-gene;luxA-gene;Salmon products;Seafood spoilage
AB An accurate amplified fragment length polymorphism (AFLP) method, including three primer sets for the selective amplification step, was developed to display the phylogenetic position of Photobacterium isolates collected from salmon products. This method was efficient for discriminating the three species Photobacterium phosphoreum, Photobacterium iliopiscarium and Photobacterium kishitanii, until now indistinctly gathered in the Photobacterium phosphoreum species group known to be strongly responsible for seafood spoilage. The AFLP fingerprints enabled the isolates to be separated into two main clusters that, according to the type strains, were assigned to the two species P. phosphoreum and P. iliopiscarium. P. kishitanii was not found in the collection. The accuracy of the method was validated by using gyrB-gene sequencing and luxA-gene PCR amplification, which confirmed the species delineation. Most of the isolates of each species were clonally distinct and even those that were isolated from the same source showed some diversity. Moreover, this AFLP method may be an excellent tool for genotyping isolates in bacterial communities and for clarifying our knowledge of the role of the different members of the Photobacterium species group in seafood spoilage.
PY 2016
PD JAN
SO International Journal Of Food Microbiology
SN 0168-1605
PU Elsevier Science Bv
VL 217
UT 000366767600014
BP 101
EP 109
DI 10.1016/j.ijfoodmicro.2015.10.018
ID 39712
ER

EF