FN Archimer Export Format
PT J
TI DNA adduct formation and induction of detoxification mechanisms in Dreissena polymorpha exposed to nitro-PAHs
BT 
AF CHATEL, Amelie
   FAUCET-MARQUIS, Virginie
   PFOHL-LESZKOWICZ, Annie
   GOURLAY-FRANCE, Catherine
   VINCENT HUBERT, Francoise
AS 1:1;2:2;3:2;4:1;5:1;
FF 1:;2:;3:;4:;5:;
C1 IRSTEA, Unite Rech Hydrosyst & Bioproc, F-92761 Antony, France.
   Univ Toulouse, INPT ENSAT, Lab Genie Chim, UMR CNRS 5503,Dept Bioproc & Syst Microbiens, F-31320 Auzeville Tolosane, France.
C2 IRSTEA, FRANCE
   UNIV TOULOUSE, FRANCE
IF 2.793
TC 24
UR https://archimer.ifremer.fr/doc/00287/39799/41532.pdf
LA English
DT Article
AB Derived polycyclic aromatic hydrocarbons (PAHs) such as nitro-PAHs are present in the environment and are known to be much more toxic than PAHs compounds. However, very few studies have analysed their effects on the aquatic environment and none have investigated the freshwater environment. In the present study, we determined whether 1-nitropyrene (1-NP), a model of nitro-PAHs, can induce DNA adducts in gills and digestive glands of the freshwater mussel Dreissena polymorpha. Two concentrations of 1-NP (50 and 500 mu M) were tested. In addition, in order to understand the metabolic pathways involved in 1-NP genotoxicity, mRNA expression of genes implicated in biotransformation mechanisms was assessed by quantitative reverse transcription-PCR. Results showed the presence of DNA adducts in both gills and digestive glands, with highest levels obtained after 5 days of exposure to 500 mu M. Metallothionein mRNA levels were enhanced in digestive glands exposed to 50 mu M. Surprisingly, at the higher concentration (500 mu M), aryl hydrocarbon receptor and HSP70 genes were only up-regulated in digestive glands while PgP mRNA levels were increased in both tissues. Results suggested a cytotoxic and genotoxic effect of 1-NP. Mussels seemed to be able to partially detoxify this compound, in view of the low amount of DNA adducts observed after 5 days exposure to 50 mu M. For the first time, 1-NP biotransformation and detoxification systems have been characterised in D. polymorpha.
PY 2014
PD NOV
SO Mutagenesis
SN 0267-8357
PU Oxford Univ Press
VL 29
IS 6
UT 000344624700009
BP 457
EP 465
DI 10.1093/mutage/geu040
ID 39799
ER

EF