Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas

Type Article
Date 2016-02
Language English
Author(s) Barranger Audrey1, 2, Heude-Berthelin Clothilde3, Rouxel Julien1, Adeline Beatrice3, Benabdelmouna Abdellah2, Burgeot Thierry1, Akcha Farida1
Affiliation(s) 1 : IFREMER, Dept Biogeochem & Ecotoxicol, Lab Ecotoxicol, Rue Ile dYeu,BP 21105, F-44311 Nantes 03, France.
2 : IFREMER, SG2M, Lab Genet & Pathol Marine Molluscs, Ave Mus Loup, F-17390 La Tremblade, France.
3 : Univ Paris 06, Unite Biol Organismes & Ecosyst Aquat BORER, Univ Caen Basse Normandie, UMR 7208,Sorbonne Univ,Museum Natl Hist Nat,IRD,C, 57 Rue Cuvier, F-75005 Paris, France.
Source Comparative Biochemistry And Physiology C-toxicology & Pharmacology (1532-0456) (Elsevier Science Inc), 2016-02 , Vol. 180 , P. 23-30
DOI 10.1016/j.cbpc.2015.11.002
WOS© Times Cited 5
Keyword(s) Crassostrea gigas, Oxidative stress, DNA damage, Germ cell, Pesticides
Abstract Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography coupled with UV and electrochemical detection analysis showed an increase in 8-oxodGuo levels in both male and female gonads after exposure to diuron. Immunohistochemistry analysis showed the presence of 8-oxodGuo at all stages of male germ cells, from early to mature stages. Conversely, the oxidized base was only present in early germ cell stages in female gonads. These results indicate that male and female genitors underwent oxidative stress following exposure to diuron, resulting in DNA oxidation in both early germ cells and gametes, such as spermatozoa, which could explain the transmission of diuron-induced DNA damage to offspring. Furthermore, immunostaining of early germ cells seems indicates that damages caused by exposure to diuron on germ line not only affect the current sexual cycle but also could affect future gametogenesis.
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Barranger Audrey, Heude-Berthelin Clothilde, Rouxel Julien, Adeline Beatrice, Benabdelmouna Abdellah, Burgeot Thierry, Akcha Farida (2016). Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas. Comparative Biochemistry And Physiology C-toxicology & Pharmacology, 180, 23-30. Publisher's official version : https://doi.org/10.1016/j.cbpc.2015.11.002 , Open Access version : https://archimer.ifremer.fr/doc/00295/40655/