FN Archimer Export Format
PT J
TI Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas
BT 
AF BARRANGER, Audrey
   HEUDE-BERTHELIN, Clothilde
   ROUXEL, Julien
   ADELINE, Beatrice
   BENABDELMOUNA, Abdellah
   BURGEOT, Thierry
   AKCHA, Farida
AS 1:1,2;2:3;3:1;4:3;5:2;6:1;7:1;
FF 1:PDG-RBE-BE-LBEX;2:;3:PDG-RBE-BE-LBEX;4:;5:PDG-RBE-SG2M-LGPMM;6:PDG-RBE-BE;7:PDG-RBE-BE-LBEX;
C1 IFREMER, Dept Biogeochem & Ecotoxicol, Lab Ecotoxicol, Rue Ile dYeu,BP 21105, F-44311 Nantes 03, France.
   IFREMER, SG2M, Lab Genet & Pathol Marine Molluscs, Ave Mus Loup, F-17390 La Tremblade, France.
   Univ Paris 06, Unite Biol Organismes & Ecosyst Aquat BORER, Univ Caen Basse Normandie, UMR 7208,Sorbonne Univ,Museum Natl Hist Nat,IRD,C, 57 Rue Cuvier, F-75005 Paris, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   MNHN, FRANCE
SI NANTES
   LA TREMBLADE
SE PDG-RBE-BE-LBEX
   PDG-RBE-SG2M-LGPMM
   PDG-RBE-BE
IN WOS Ifremer jusqu'en 2018
   copubli-france
IF 2.416
TC 11
UR https://archimer.ifremer.fr/doc/00295/40655/39753.pdf
LA English
DT Article
DE ;Crassostrea gigas;Oxidative stress;DNA damage;Germ cell;Pesticides
AB Chemical pollution by pesticides has been identified as a possible contributing factor to the massive mortality outbreaks observed in Crassostrea gigas for several years. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to the herbicide diuron at environmental concentrations during gametogenesis. This trans-generational effect occurs through damage to genitor-exposed gametes, as measured by the comet-assay. The presence of DNA damage in gametes could be linked to the formation of DNA damage in other germ cells. In order to explore this question, the levels and cell distribution of the oxidized base lesion 8-oxodGuo were studied in the gonads of exposed genitors. High-performance liquid chromatography coupled with UV and electrochemical detection analysis showed an increase in 8-oxodGuo levels in both male and female gonads after exposure to diuron. Immunohistochemistry analysis showed the presence of 8-oxodGuo at all stages of male germ cells, from early to mature stages. Conversely, the oxidized base was only present in early germ cell stages in female gonads. These results indicate that male and female genitors underwent oxidative stress following exposure to diuron, resulting in DNA oxidation in both early germ cells and gametes, such as spermatozoa, which could explain the transmission of diuron-induced DNA damage to offspring. Furthermore, immunostaining of early germ cells seems indicates that damages caused by exposure to diuron on germ line not only affect the current sexual cycle but also could affect future gametogenesis.
PY 2016
PD FEB
SO Comparative Biochemistry And Physiology C-toxicology & Pharmacology
SN 1532-0456
PU Elsevier Science Inc
VL 180
UT 000369878900003
BP 23
EP 30
DI 10.1016/j.cbpc.2015.11.002
ID 40655
ER

EF