FN Archimer Export Format PT J TI Crassaminicella profunda gen. nov., sp nov., an anaerobic marine bacterium isolated from deep-sea sediments BT AF LAKHAL, Raja PRADEL, Nathalie POSTEC, Anne IVIER, Bernard CAYOL, Jean-Luc GODFROY, Anne FARDEAU, Marie-Laure GALES, Gregoire AS 1:1;2:1;3:1;4:1;5:1;6:2;7:1;8:1,3; FF 1:;2:;3:;4:;5:;6:PDG-REM-EEP-LMEE;7:;8:; C1 Aix Marseille Univ, Univ Sud ToulonVar, CNRS INSU, IRD,MIO,UM110, F-13288 Marseille 09, France. UBO, CNRS, IFREMER, Labe Microbiol Environm Extremes,UMR 6197, F-29280 Plouzane, France. Aix Marseille Univ, CEREGE, UMR 7330, F-13331 Marseille, France. C2 UNIV AIX MARSEILLE, FRANCE IFREMER, FRANCE UNIV AIX MARSEILLE, FRANCE SI BREST SE PDG-REM-EEP-LMEE UM BEEP-LM2E IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.439 TC 11 UR https://archimer.ifremer.fr/doc/00296/40742/39992.pdf LA English DT Article CR BIG BO L'Atalante AB A novel, anaerobic, chemo-organotrophic bacterium, designated strain Ra1766HT, was isolated from sediments of the Guaymas basin (Gulf of California, Mexico) taken from a depth of 2002 m. Cells were thin, motile, Gram-stain-positive, flexible rods forming terminal endospores. Strain Ra1766H(T) grew at temperatures of 25-45 degrees C (optimum 30 degrees C), pH 6.7-8.1 (optimum 7.5) and in a salinity of 5-60 g l(-1) NaCl (optimum 30 g l(-1)). It was an obligate heterotrophic bacterium fermenting carbohydrates (glucose and mannose) and organic acids (pyruvate and succinate). Casamino acids and amino acids (glutamate, aspartate and glycine) were also fermented. The main end products from glucose fermentation were acetate, butyrate, ethanol, H-2 and CO2. Sulfate, sulfite, thiosulfate, elemental sulfur, fumarate, nitrate, nitrite and Fe(III) were not used as terminal electron acceptors. The predominant cellular fatty acids were C-14 : 0, C-16:1 omega 7, C-16:1 omega 7 DMA and C-16:0. The main polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipids. The G +C content of the genomic DNA was 33.7 molo/o. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Ra1766H(T) was affiliated to cluster XI of the order Clostridia les, phylum Firmicutes. The closest phylogenetic relative of Ra1766H(T) was Geosporobacter subterraneus (94.2% 16S rRNA gene sequence similarity). On the basis of phylogenetic inference and phenotypic properties, strain Ra1766H(T) (=DSM 27501(T)=JCM 19377(T)) is proposed to be the type strain of a novel species of a novel genus, named Crassaminicella pro funda. PY 2015 PD SEP SO International Journal Of Systematic And Evolutionary Microbiology SN 1466-5026 PU Soc General Microbiology VL 65 UT 000364108400048 BP 3097 EP 3102 DI 10.1099/ijsem.0.000386 ID 40742 ER EF