FN Archimer Export Format
PT J
TI Detection and distribution of ostreid herpesvirus 1 in experimentally infected Pacific oyster spat
BT 
AF SEGARRA, Amelie
   BAILLON, Laury
   FAURY, Nicole
   TOURBIEZ, Delphine
   RENAULT, Tristan
AS 1:1;2:1;3:1;4:1;5:1;
FF 1:PDG-RBE-SG2M-LGPMM;2:PDG-RBE-SG2M-LGPMM;3:PDG-RBE-SG2M-LGPMM;4:PDG-RBE-SG2M-LGPMM;5:PDG-RBE;
C1 IFREMER, LGPMM, Unite Sante Genet & Microbiol Mollusques SG2M, F-17390 La Tremblade, France.
C2 IFREMER, FRANCE
SI LA TREMBLADE
   NANTES
SE PDG-RBE-SG2M-LGPMM
   PDG-RBE
IN WOS Ifremer jusqu'en 2018
IF 2.379
TC 21
UR https://archimer.ifremer.fr/doc/00300/41099/40272.pdf
LA English
DT Article
DE ;Crassostrea gigas;In situ hybridization;Ostreid herpesvirus 1;Viral DNA;Viral RNA
AB High mortality rates are reported in spat and larvae of Pacific oyster Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in France. Although the viral infection has been experimentally reproduced in oyster larvae and spat, little knowledge is currently available concerning the viral entry and its distribution in organs and tissues. This study compares OsHV-1 DNA and RNA detection and localization in experimentally infected oysters using two virus doses: a low dose that did not induce any mortality and a high dose inducing high mortality. Real time PCR demonstrated significant differences in terms of viral DNA amounts between the two virus doses. RNA transcripts were detected in oysters receiving the highest dose of viral suspension whereas no transcript was observed in oysters injected with the low dose. This study also allowed observing kinetics of viral DNA and RNA detection in different tissues of oyster spat. Finally, viral detection was significantly different in function of tissues (p < 0.005), time (p < 0.005) with an interaction between tissues and time (p < 0.005) for each probe.
PY 2016
PD JAN
SO Journal Of Invertebrate Pathology
SN 0022-2011
PU Academic Press Inc Elsevier Science
VL 133
UT 000368317200009
BP 59
EP 65
DI 10.1016/j.jip.2015.11.013
ID 41099
ER

EF