FN Archimer Export Format
PT J
TI Low incidence of clonality in cold water corals revealed through the novel use of standardized protocol adapted to deep sea sampling
BT 
AF BECHELER, Ronan
   CASSONE, Anne-Laure
   NOEL, Philippe
   MOUCHEL, Olivier
   MORRISON, Cheryl L.
   ARNAUD-HAOND, Sophie
AS 1:1;2:1;3:1;4:1;5:3;6:1,2;
FF 1:PDG-REM-EEP-LEP;2:;3:PDG-REM-EEP-LEP;4:;5:;6:PDG-RBE-MARBEC-LHM;
C1 IFREMER, Ctr Brest, EEP LEP, Site Technopole, F-29480 Plouzane, France.
   IFREMER, Ctr Sete, UMR MARBEC, Bd Jean Monnet,BP 171, F-34203 Sete, France.
   US Geol Survey, Leetown Sci Ctr, Natl Fish Hlth Res Lab, 11649 Leetown Rd, Kearneysville, WV 25430 USA.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
   US GEOL SURVEY, USA
SI BREST
   SETE
SE PDG-REM-EEP-LEP
   PDG-RBE-MARBEC-LHM
UM MARBEC
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
IF 2.451
TC 8
UR https://archimer.ifremer.fr/doc/00300/41117/40291.pdf
LA English
DT Article
CR BOBECO LEG1
   BOBECO LEG2
BO Unknown
   Pourquoi pas ?
DE ;Cold-water coral;Lophelia pertusa;Madrepora oculata;Clonality;Fine-grained spatial genetic structure;Standardized sampling
AB Sampling in the deep sea is a technical challenge, which has hindered the acquisition of robust datasets that are necessary to determine the fine-grained biological patterns and processes that may shape genetic diversity. Estimates of the extent of clonality in deep-sea species, despite the importance of clonality in shaping the local dynamics and evolutionary trajectories, have been largely obscured by such limitations. Cold-water coral reefs along European margins are formed mainly by two reef-building species, Lophelia pertusa and Madrepora oculata. Here we present a fine-grained analysis of the genotypic and genetic composition of reefs occurring in the Bay of Biscay, based on an innovative deep-sea sampling protocol. This strategy was designed to be standardized, random, and allowed the georeferencing of all sampled colonies. Clonal lineages discriminated through their Multi-Locus Genotypes (MLG) at 6 to 7 microsatellite markers could thus be mapped to assess the level of clonality and the spatial spread of clonal lineages. High values of clonal richness were observed for both species across all sites suggesting a limited occurrence of clonality, which likely originated through fragmentation. Additionally, spatial autocorrelation analysis underlined the possible occurrence of fine-grained genetic structure in several populations of both L. pertusa and M. oculata. The two cold-water coral species examined had contrasting patterns of connectivity among canyons, with among-canyon genetic structuring detected in M. oculata, whereas L. pertusa was panmictic at the canyon scale. This study exemplifies that a standardized, random and georeferenced sampling strategy, while challenging, can be applied in the deep sea, and associated benefits outlined here include improved estimates of fine grained patterns of clonality and dispersal that are comparable across sites and among species.
PY 2017
PD NOV
SO Deep-sea Research Part Ii-topical Studies In Oceanography
SN 0967-0645
PU Pergamon-elsevier Science Ltd
VL 145
UT 000418985900011
BP 120
EP 130
DI 10.1016/j.dsr2.2015.11.013
ID 41117
ER

EF