FN Archimer Export Format
PT J
TI Ontogeny of osmoregulation in the Pacific blue shrimp, Litopenaeus stylirostris (decapoda, penaeidae): Deciphering the role of the Na+/K+-ATPase
BT 
AF PHAM, Dominique
   CHARMANTIER, Guy
   BOULO, Viviane
   WABETE, Nelly
   ANSQUER, Dominique
   DAUGA, Clement
   GROUSSET, Evelyse
   LABREUCHE, Yannick
   CHARMANTIER-DAURES, Mireille
AS 1:1;2:2;3:1,2;4:1;5:1;6:1;7:2;8:3,4;9:2;
FF 1:PDG-RBE-LEADNC;2:;3:PDG-RBE-LEADNC;4:PDG-RBE-LEADNC;5:PDG-RBE-LEADNC;6:;7:;8:PDG-RBE-PFOM;9:;
C1 IFREMER, Lagon, Environm & Aquaculture Durable, Boulouparis, Nouvelle Caledo, France.
   Univ Montpellier, IFREMER, Adaptat Ecophysiol & Ontogenese, Marbec,UM,CNRS,IRD,UMR 9190, Pl Eugene Bataillon,CC 092, F-34095 Montpellier 05, France.
   IFREMER, Unite Physiol Fonct Organismes Marins, ZI Pointe Diable, CS 10070, F-29280 Plouzane, France.
   Univ Paris 06, Univ Paris 04, Stn Biol Roscoff, Integrat Biol Marine Models,CNRS,UMR 8227, CS 90074, F-29688 Roscoff, France.
C2 IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
   IFREMER, FRANCE
   UNIV PARIS 06, FRANCE
SI SAINT VINCENT
   ROSCOFF
SE PDG-RBE-LEADNC
   PDG-RBE-PFOM
UM MARBEC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 1.757
TC 15
UR https://archimer.ifremer.fr/doc/00311/42176/41477.pdf
LA English
DT Article
DE ;Penaeid;Litopenaeus stylirostris;Ontogeny;Osmoregulation;Na+/K+-ATPase;RNA interference
AB The role of the main ion transporting enzyme Na +/K + − ATPase in osmoregulation processes was investigated in Litopenaeus stylirostris. The development and localization of the osmoregulation sites were studied during ontogenesis by immunodetection of Na+ K+-ATPase using monoclonal antibodies and transmission electron microscopy (TEM). Osmoregulation sites were identified as the pleurae and branchiostegites in the zoeae and mysis stages. In the subsequent post-metamorphic stages the osmoregulatory function was mainly located in the epipodites and branchiostegites and osmotic regulation was later detected in the gills. The presence of ionocytes and microvilli in these tissues confirmed their role in ionic processes. The complete open reading frame of the mRNA coding for the α-subunit of Na + K + − ATPase was characterized in L. stylirostris. The resulting 3092-bp cDNA (LsNKA) encodes a putative 1011-amino-acid protein with a predicted molecular mass of 112.3 kDa. The inferred amino acid sequence revealed that the putative protein possesses the main structural characteristics of the Na + K + − ATPase α-subunits.  Quantitative RT-PCR analyses indicated that LsNKA transcripts did not significantly vary between the different developmental stages. The number of transcripts was about 2.5-fold higher in the epipodites and gills than in any other tissues tested in juveniles. A reverse genetic approach was finally implemented to study the role of LsNKA in vivo. Knock down of LsNKA expression by gene-specific dsRNA injection led to an increase of shrimp mortality following an abrupt salinity change compared to control animals. These data strongly suggest that LsNKA plays an important role in osmoregulation when the shrimp are challenged by changing salinities.
PY 2016
PD JUL
SO Comparative Biochemistry And Physiology B-biochemistry & Molecular Biology
SN 1096-4959
PU Elsevier Science Inc
VL 196
UT 000376705300004
BP 27
EP 37
DI 10.1016/j.cbpb.2015.12.007
ID 42176
ER

EF