TY - JOUR T1 - Oyster reproduction is affected by exposure to polystyrene microplastics A1 - Sussarellu,Rossana A1 - Suquet,Marc A1 - Thomas,Yoann A1 - Lambert,Christophe A1 - Fabioux,Caroline A1 - Pernet,Marie Eve Julie A1 - Le Goic,Nelly A1 - Quillien,Virgile A1 - Mingant,Christian A1 - Epelboin,Yanouk A1 - Corporeau,Charlotte A1 - Guyomarch,Julien A1 - Robbens,Johan A1 - Paul-Pont,Ika A1 - Soudant,Philippe A1 - Huvet,Arnaud AD - CNRS, Inst Francais Rech Exploitat Mer, Inst Rech Dev, Lab Sci Environm Marin,UMR 6539 UBO, F-29280 Plouzane, France. AD - Ctr Documentat Rech Experimentat, F-29218 Brest, France. AD - Inst Poor Landbouw Visserijonderzoek, B-8400 Oostende, Belgium. AD - Inst Francais Rech Exploitat Mer, Lab Ecotoxicol, Dept Biogeochem & Ecotoxicol, F-43110 Nantes, France. UR - https://doi.org/10.1073/pnas.1519019113 DO - 10.1073/pnas.1519019113 KW - microplastic KW - reproduction KW - energy allocation KW - oyster N2 - Plastics are persistent synthetic polymers that accumulate as waste in the marine environment. Microplastic (MP) particles are derived from the breakdown of larger debris or can enter the environment as microscopic fragments. Because filter-feeder organisms ingest MP while feeding, they are likely to be impacted by MP pollution. To assess the impact of polystyrene microspheres (micro-PS) on the physiology of the Pacific oyster, adult oysters were experimentally exposed to virgin micro-PS (2 and 6 µm in diameter; 0.023 mg·L−1) for 2 mo during a reproductive cycle. Effects were investigated on ecophysiological parameters; cellular, transcriptomic, and proteomic responses; fecundity; and offspring development. Oysters preferentially ingested the 6-µm micro-PS over the 2-µm-diameter particles. Consumption of microalgae and absorption efficiency were significantly higher in exposed oysters, suggesting compensatory and physical effects on both digestive parameters. After 2 mo, exposed oysters had significant decreases in oocyte number (−38%), diameter (−5%), and sperm velocity (−23%). The D-larval yield and larval development of offspring derived from exposed parents decreased by 41% and 18%, respectively, compared with control offspring. Dynamic energy budget modeling, supported by transcriptomic profiles, suggested a significant shift of energy allocation from reproduction to structural growth, and elevated maintenance costs in exposed oysters, which is thought to be caused by interference with energy uptake. Molecular signatures of endocrine disruption were also revealed, but no endocrine disruptors were found in the biological samples. This study provides evidence that micro-PS cause feeding modifications and reproductive disruption in oysters, with significant impacts on offspring. Y1 - 2016/03 PB - Natl Acad Sciences JF - Proceedings Of The National Academy Of Sciences Of The United States Of America SN - 0027-8424 VL - 113 IS - 9 SP - 2430 EP - 2435 ID - 42233 ER -