Osmoregulation in larvae and juveniles of two recently separated Macrobrachium species: Expression patterns of ion transporter genes

Type Article
Date 2016-05
Language English
Author(s) Boudour-Boucheker Nesrine1, Boulo VivianeORCID2, Charmantier-Daures Mireille1, Anger Klaus3, Charmantier Guy1, Lorin-Nebel Catherine1
Affiliation(s) 1 : Univ Montpellier, Adaptat Ecophysiol & Ontogenese, MARBEC UMR9190, Cc 092,Pl E Bataillon, F-34095 Montpellier 05, France.
2 : IFREMER, LEAD, BP2059, Noumea 98846, New Caledonia.
3 : Biol Anstalt Helgoland, Alfred Wegener Inst Polar & Meeresforsch, D-27498 Helgoland, Germany.
Source Comparative Biochemistry And Physiology A-molecular & Integrative Physiology (1095-6433) (Elsevier Science Inc), 2016-05 , Vol. 195 , P. 39-45
DOI 10.1016/j.cbpa.2016.02.005
WOS© Times Cited 12
Keyword(s) Salinity, Crustaceans, Branchial chamber, NHE3 transporter, V-type H+-ATPase, Na+/K+-ATPase
Abstract In this comparative study, osmoregulatory mechanisms were analyzed in two closely related species of palaemonid shrimp from Brazil, Macrobrachium pantanalense and Macrobrachium amazonicum. A previous investigation showed that all postembryonic stages of M. pantanalense from inland waters of the Pantanal are able to hyper-osmoregulate in fresh water, while this species was not able to hypo-osmoregulate at high salinities. In M. amazonicum originating from the Amazon estuary, in contrast, all stages are able to hypo-osmoregulate, but only first-stage larvae, late juveniles and adults are able to hyper-osmoregulate in fresh water. The underlying molecular mechanisms of these physiological differences have not been known. We therefore investigated the expression patterns of three ion transporters (NKA α-subunit, VHA B-subunit and NHE3) following differential salinity acclimation in different ontogenetic stages (stage-V larvae, juveniles) of both species. Larval NKAα expression was at both salinities significantly higher in M. pantanalense than in M. amazonicum, whereas no difference was noted in juveniles. VHA was also more expressed in larvae of M. pantanalense than in those of M. amazonicum. When NHE3 expression is compared between the larvae of the two species, further salinity-related differences were observed, with generally higher expression in the inland species. Overall, a high expression of ion pumps in M. pantanalense suggests an evolutionary key role of these transporters in freshwater invasion.
Full Text
File Pages Size Access
7 547 KB Access on demand
Author's final draft 23 197 KB Open access
Top of the page

How to cite 

Boudour-Boucheker Nesrine, Boulo Viviane, Charmantier-Daures Mireille, Anger Klaus, Charmantier Guy, Lorin-Nebel Catherine (2016). Osmoregulation in larvae and juveniles of two recently separated Macrobrachium species: Expression patterns of ion transporter genes. Comparative Biochemistry And Physiology A-molecular & Integrative Physiology, 195, 39-45. Publisher's official version : https://doi.org/10.1016/j.cbpa.2016.02.005 , Open Access version : https://archimer.ifremer.fr/doc/00313/42427/