FN Archimer Export Format
PT J
TI Block Copolymer/DNA Vaccination Induces a Strong Allergen-Specific Local Response in a Mouse Model of House Dust Mite Asthma
BT 
AF ROLLAND-DEBORD, Camille
   LAIR, David
   ROUSSEY-BIHOUEE, Tiphaine
   HASSOUN, Dorian
   EVRARD, Justine
   CHEMINANT, Marie-Aude
   CHESNE, Julie
   BRAZA, Faouzi
   MAHAY, Guillaume
   PORTERO, Vincent
   SAGAN, Christine
   PITARD, Bruno
   MAGNAN, Antoine
AS 1:1,2;2:1,2;3:1,2;4:1,2;5:1;6:1,2;7:1,2;8:1,2;9:1,2;10:1,2;11:3;12:1;13:1,2;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;
C1 Ctr Natl Rech Sci 6291, INSERM, U1087, Unite Mixte Rech, Nantes, France.
   Univ Nantes, Nantes, France.
   Ctr Hosp Univ, Serv Anat & Cytol Pathol, Nantes, France.
C2 CNRS, FRANCE
   UNIV NANTES, FRANCE
   CHU NANTES, FRANCE
IN DOAJ
IF 3.234
TC 10
UR https://archimer.ifremer.fr/doc/00315/42621/41998.pdf
   https://archimer.ifremer.fr/doc/00315/42621/41999.docx
LA English
DT Article
AB Background: Allergic asthma is caused by abnormal immunoreactivity against allergens such as house dust mites among which Dermatophagoides farinae (Der f) is a common species. Currently, immunotherapy is based on allergen administration, which has variable effect from patient to patient and may cause serious side effects, principally the sustained risk of anaphylaxis. DNA vaccination is a promising approach by triggering a specific immune response with reduced allergenicity. Objective: The aim of the study is to evaluate the effects of DNA immunization with Der f1 allergen specific DNA on allergic sensitization, inflammation and respiratory function in mice. Methods: Mice were vaccinated 28 and 7 days before allergen exposure with a Der f1-encoding plasmid formulated with a block copolymer. Asthma was induced by skin sensitization followed by intra-nasal challenges with Der f extract. Total lung, broncho-alveolar lavage (BAL) and spleen cells were analyzed by flow cytometry for their surface antigen and cytokine expression. Splenocytes and lung cell IFN-gamma production by CD8+ cells in response to Der f CMH1-restricted peptides was assessed by ELISPOT. IgE, IgG1 and IgG2a were measured in serum by ELISA. Specific bronchial hyperresponsiveness was assessed by direct resistance measurements. Results: Compared to animals vaccinated with an irrelevant plasmid, pVAX-Der f1 vaccination induced an increase of B cells in BAL, and an elevation of IL-10 and IFN-gamma but also of IL-4, IL-13 and IL-17 producing CD4+ lymphocytes in lungs and of IL-4 and IL-5 in spleen. In response to CD8-restricted peptides an increase of IFN-gamma was observed among lung cells. IgG2a levels non-specifically increased following block copolymer/DNA vaccination although IgE, IgG1 levels and airways resistances were not impacted. Conclusions & Clinical Relevance: DNA vaccination using a plasmid coding for Der f1 formulated with the block copolymer 704 induces a specific immune response in the model of asthma used herein.
PY 2014
PD JAN
SO Plos One
SN 1932-6203
PU Public Library Science
VL 9
IS 1
UT 000330621900014
DI 10.1371/journal.pone.0085976
ID 42621
ER

EF