FN Archimer Export Format PT J TI A Shift from Cellular to Humoral Responses Contributes to Innate Immune Memory in the Vector Snail Biomphalaria glabrata BT AF PINAUD, Silvain PORTELA, Julien DUVAL, David NOWACKI, Fanny C. OLIVE, Marie-Aude ALLIENNE, Jean-Francois GALINIER, Richard DHEILLY, Nolwenn M. KIEFFER-JAQUINOD, Sylvie MITTA, Guillaume THERON, Andre GOURBAL, Benjamin AS 1:1;2:1;3:1;4:1,2;5:1;6:1;7:1;8:1,3;9:4;10:1;11:1;12:1; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:; C1 Univ Montpellier, IFREMER, CNRS, Univ Perpignan,IHPE UMR 5244, Via Domitia, Perpignan, France. Aberystwyth Univ, Inst Biol Environm & Rural Sci, Aberystwyth Ceredigion, Scotland. SUNY Stony Brook, Sch Marine & Atmospher Sci, Stony Brook, NY 11794 USA. INSERM CEA UJF CEA, Lab Biol Grande Echelle UMR S 1038, Plate Forme Analyses Proteom EDyP Serv, Grenoble, France. C2 UNIV MONTPELLIER, FRANCE UNIV ABERYSTWYTH, UK SUNY STONY BROOK, USA CEA, FRANCE UM IHPE IN DOAJ IF 6.608 TC 89 UR https://archimer.ifremer.fr/doc/00315/42664/71872.pdf https://archimer.ifremer.fr/doc/00315/42664/71873.tif https://archimer.ifremer.fr/doc/00315/42664/71874.xlsx https://archimer.ifremer.fr/doc/00315/42664/71875.xlsx https://archimer.ifremer.fr/doc/00315/42664/71876.xlsx https://archimer.ifremer.fr/doc/00315/42664/71877.pdf https://archimer.ifremer.fr/doc/00315/42664/71878.pdf LA English DT Article AB Discoveries made over the past ten years have provided evidence that invertebrate antiparasitic responses may be primed in a sustainable manner, leading to the failure of a secondary encounter with the same pathogen. This phenomenon called "immune priming" or "innate immune memory" was mainly phenomenological. The demonstration of this process remains to be obtained and the underlying mechanisms remain to be discovered and exhaustively tested with rigorous functional and molecular methods, to eliminate all alternative explanations. In order to achieve this ambitious aim, the present study focuses on the Lophotrochozoan snail, Biomphalaria glabrata, in which innate immune memory was recently reported. We provide herein the first evidence that a shift from a cellular immune response (encapsulation) to a humoral immune response (biomphalysin) occurs during the development of innate memory. The molecular characterisation of this process in Biomphalaria/Schistosoma system was undertaken to reconcile mechanisms with phenomena, opening the way to a better comprehension of innate immune memory in invertebrates. This prompted us to revisit the artificial dichotomy between innate and memory immunity in invertebrate systems. PY 2016 PD JAN SO Plos Pathogens SN 1553-7366 PU Public Library Science VL 12 IS 1 UT 000369374500019 DI 10.1371/journal.ppat.1005361 ID 42664 ER EF