FN Archimer Export Format PT J TI Metabolic responses of clam Ruditapes philippinarum exposed to its pathogen Vibrio tapetis in relation to diet BT AF RICHARD, Gaelle GUERARD, Fabienne CORPOREAU, Charlotte LAMBERT, Christophe PAILLARD, Christine PERNET, Fabrice AS 1:1;2:1;3:2;4:1;5:1;6:2; FF 1:;2:;3:PDG-RBE-PFOM-PI;4:;5:;6:PDG-RBE-PFOM-PI; C1 Univ Bretagne Occidentale, Inst Univ Europeen Mer, LEMAR IUEM UBO, UMR CNRS UBO IRD IFREMER 6539, Technopole Brest Iroise,Rue Dumont dUrville, F-29280 Plouzane, France. IFREMER, UMR LEMAR CNRS UBO IRD Ifremer 6539, Technopole Brest Iroise CS 10070, F-29280 Plouzane, France. C2 UBO, FRANCE IFREMER, FRANCE SI BREST SE PDG-RBE-PFOM-PI UM LEMAR IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 3.218 TC 7 UR https://archimer.ifremer.fr/doc/00316/42684/42057.pdf LA English DT Article DE ;Antioxidant enzymes;Immunity;Energetic metabolism;Ruditapes philippinarum;Vibrio tapetis AB We investigated the effect of brown ring disease (BRD)1 development and algal diet on energy reserves and activity of enzymes related to energy metabolism, antioxidant system and immunity in Manila clam, Ruditapes philippinarum. We found that algal diet did not impact the metabolic response of clams exposed to Vibrio tapetis. At two days post-injection (dpi), activities of superoxide dismutase and glutathione peroxidase (GPx) decreased whereas activities of nitric oxide synthase (iNOS) and catalase increased in infected clams, although no clinical signs were visible (BRD-). At 7 dpi, activities of several antioxidant and immune-related enzymes were markedly increased in BRD-likely indicating an efficient reactive oxygen species (ROS) scavenging compared to animals which developed clinical signs of BRD (BRD+). Therefore, resistance to BRD clinical signs appearance was associated with higher detoxification of ROS and enhancement of immune response. This study provides new biochemical indicators of disease resistance and a more comprehensive view of the global antioxidant response of clam to BRD development. PY 2016 PD JUN SO Developmental And Comparative Immunology SN 0145-305X PU Elsevier Sci Ltd VL 60 UT 000374613800010 BP 96 EP 107 DI 10.1016/j.dci.2016.02.026 ID 42684 ER EF