FN Archimer Export Format
PT J
TI The quality of great scallop (Pecten maximus) sperm after thawing
BT 
AF SUQUET, Marc
   GOURTAY, Clemence
   DONVAL, Anne
   LE GOIC, Nelly
   QUERE, Claudie
   MALO, Florent
   LE GRAND, Jacqueline
   RATISKOL, Dominique
   MINGANT, Christian
   FAUVEL, Christian
AS 1:1;2:1;3:2;4:2;5:3;6:1;7:1;8:1;9:1;10:4;
FF 1:PDG-RBE-PFOM-PI;2:PDG-RBE-PFOM-ARN;3:;4:;5:PDG-RBE-PFOM-PI;6:PDG-RBE-LEADNC;7:PDG-RBE-PFOM-PI;8:PDG-RBE-PFOM-PI;9:PDG-RBE-PFOM-PI;10:PDG-RBE-MARBEC-L3AS;
C1 IFREMER, PFOM Dept, Stn Expt Argenton, UMR 6539, Argenton, France.
   IUEM, Lemar, UMR 6539, Technopole Brest Iroise, Plouzane, France.
   IFREMER, PFOM Dept, UMR 6539, Technopole Brest Iroise, Plouzane, France.
   IFREMER, Marbec, Stn Ifremer, UMR 248, Palavas Les Flots, France.
C2 IFREMER, FRANCE
   UBO, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI ARGENTON
   BREST
   NOUMEA
   PALAVAS
SE PDG-RBE-PFOM-PI
   PDG-RBE-PFOM-ARN
   PDG-RBE-LEADNC
   PDG-RBE-MARBEC-L3AS
UM LEMAR
   MARBEC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.585
TC 9
UR https://archimer.ifremer.fr/doc/00317/42862/43912.pdf
LA English
DT Article
DE ;Pecten maximus;Reproduction;Gamete;Sperm;Cryopreservation
AB Most publications devoted to the cryopreservation of mollusc sperm have focused on the definition of technical protocols, avoiding the description of sperm quality after thawing. The present study investigated the effects of cryopreservation on sperm quality in the great scallop. Wild scallop were fished during the natural spawning period and conditioned in the hatchery before use. Sperm samples were obtained after intragonadal injection of serotonin and cryopreserved using a previously published protocol.  Sperm quality was assessed using a panel of four parameters: sperm motility characteristics, using a a computer assisted sperm analysis plugin with Image J, intracellular ATP content using an ATP-Lite kit, sperm integrity, using flow cytometry and sperm morphology, using transmission electron microscopy. For each parameter, fresh (control) and thawed spermatozoa were compared.  A significant decrease of both the percentage of motile spermatozoa (reduction: 75%) and sperm swimming speed (86%) were observed for thawed sperm compared with fresh sperm. The percentage of living spermatozoa, as assessed using flow cytometry, was significantly lower for thawed sperm (72.4 ± 2.5%) compared with fresh sperm (86.4 ± 1.1). However, no significant difference of intracellular sperm ATP content was observed between fresh and thawed sperm. Post thawing, while some spermatozoa showed little or no morphological differences compared with fresh sperm, others had undergone drastic changes, including swelling of the plasma membrane, structural alterations of the chromatin and damage to mitochondria.  In conclusion, the descriptive parameters studied in the present work showed that the quality of thawed great scallop sperm was lower than that of fresh cells but was still sufficient for use in aquaculture programs and sperm cryobanking for this species.
PY 2016
PD APR
SO General And Comparative Endocrinology
SN 0016-6480
PU Academic Press Inc Elsevier Science
VL 229
UT 000375734800015
BP 127
EP 131
DI 10.1016/j.ygcen.2016.02.023
ID 42862
ER

EF