Transcriptional changes in Crassostrea gigas oyster spat following a parental exposure to the herbicide diuron

Type Article
Date 2016-06
Language English
Author(s) Rondon R.1, 2, Akcha Farida3, Alonso P.1, Menard Dominique3, Rouxel Julien3, Montagnani Caroline1, Mitta Guillaume2, Cosseau C.2, Grunau C.2
Affiliation(s) 1 : Univ Montpellier, CNRS, IFREMER, IHPE UMR 5244, Univ Perpignan Via Domitia, F-34095 Montpellier 5, France.
2 : Univ Montpellier, IFREMER, CNRS, IHPE UMR 5244, Univ Perpignan Via Domitia, F-66860 Perpignan, France.
3 : IFREMER, Dept Biogeochem & Ecotoxicol, Lab Ecotoxicol, Rue Ile dYeu,BP 21105, F-44311 Nantes 03, France.
Source Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2016-06 , Vol. 175 , P. 47-55
DOI 10.1016/j.aquatox.2016.03.007
WOS© Times Cited 11
Keyword(s) Mollusk, Oyster, Diuron, Transcriptomic, Ecotoxicology
Abstract The Pacific oyster Crassostrea gigas is the main oyster species produced in the world, and a key coastal economic resource in France. High mortalities affect Pacific oysters since 2008 in France and Europe. Their origins have been attributed to a combination of biotic and abiotic factors, underlining the importance of environment quality. The impact of water pollution has been pointed out and one of the pollutants, the genotoxic herbicide diuron, occurs at high concentrations all along the French coasts. Previous work has revealed that a parental exposure to diuron had a strong impact on hatching rates and offspring development even if spats were not exposed to diuron themselves. In this study, we explored for the first time the transcriptional changes occurring in oyster spats (non exposed) originating from genitors exposed to an environmentally relevant concentration of diuron during gametogenesis using the RNAseq methodology. We identified a transcriptomic remodeling revealing an effect of the herbicide. Different molecular pathways involved in energy production, translation and cell proliferation are particularly disturbed. This analysis revealed modulated candidate genes putatively involved in response to oxidative stress and mitochondrial damage in offspring of genitors exposed to diuron. Complementary measures of the activity of enzymes involved in these latter processes corroborate the results obtained at the transcriptomic level. In addition, our results suggested an increase in energy production and mitotic activity in 5-month-spats from diuron-exposed genitors. These results could correspond to a "catch-up growth" phenomenon allowing the spats from diuron-exposed genitors, which displayed a growth delay at 3 months, to gain a normal size when they reach the age of 6 months. These results indicate that exposure to a concentration of diuron that is frequently encountered in the field during the oyster’s gametogenesis stage can impact the next generation and may result in fitness disturbance.
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