FN Archimer Export Format
PT J
TI De novo assembly and annotation of the European abalone Haliotis tuberculata transcriptome
BT 
AF HARNEY, Ewan
   DUBIEF, Bruno
   BOUDRY, Pierre
   BASUYAUX, Olivier
   SCHILHABEL, Markus B.
   HUCHETTE, Sylvain
   PAILLARD, Christine
   NUNES, Flavia
AS 1:1;2:1;3:2;4:3;5:4;6:5;7:1;8:1;
FF 1:;2:;3:PDG-RBE-PFOM;4:;5:;6:;7:;8:;
C1 UEB, Lab Sci Environm Marin LEMAR, Inst Univ Europeen Mer, UMR6539,CNRS,UBO,IRD,Ifremer, Pl Nicolas Copernic, F-29280 Plouzane, France.
   UBO, IFREMER, Lab Sci Environm Marin LEMAR, UMR 6539,CNRS,IRD,Ctr Bretagne ZI, F-29280 Plouzane, France.
   Ctr Expt, SMEL, F-50560 Blainville Sur Mer, France.
   Univ Kiel, Inst Clin Mol Biol, D-24105 Kiel, Germany.
   France Haliotis, F-29880 Kerazan Lilia, Plouguemeau, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
   SMEL, FRANCE
   UNIV KIEL, GERMANY
   FRANCE HALIOTIS, FRANCE
SI BREST
SE PDG-RBE-PFOM
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 1.923
TC 31
UR https://archimer.ifremer.fr/doc/00318/42940/45103.pdf
LA English
DT Article
DE ;RNA-Seq;Mollusca;Gastropoda;Development;Vibrio harveyi;Climate change
AB The European abalone Haliotis tuberculata is a delicacy and consequently a commercially valuable gastropod species. Aquaculture production and wild populations are subjected to multiple climate-associated stressors and anthropogenic pressures, including rising sea-surface temperatures, ocean acidification and an emerging pathogenic Vibrio infection. Transcript expression data provides a valuable resource for understanding abalone responses to variation in the biotic and abiotic environment. To generate an extensive transcriptome, we performed next-generation sequencing of RNA on larvae exposed to temperature and pH variation and on haemolymph of adults from two wild populations after experimental infection with Vibrio harveyi. We obtained more than 1.5 billion raw paired-end reads, which were assembled into 328,519 contigs. Filtration and clustering produced a transcriptome of 41,099 transcripts, of which 10,626 (25.85%) were annotated with Blast hits, and 7380 of these were annotated with Gene Ontology (GO) terms in Blast2Go. A differential expression analysis comparing all samples from the two life stages identified 5690 and 10,759 transcripts with significantly higher expression in larvae and adult haemolymph respectively. This is the greatest sequencing effort yet in the Haliotis genus, and provides the first high-throughput transcriptomic resource for H. tuberculata
PY 2016
PD AUG
SO Marine Genomics
SN 1874-7787
PU Elsevier Science Bv
VL 28
UT 000382271800004
BP 11
EP 16
DI 10.1016/j.margen.2016.03.002
ID 42940
ER

EF