Microalgal sensitivity varies between a diuron-resistant strain and two wild strains when exposed to diuron and irgarol, alone and in mixtures

Type Article
Date 2016-05
Language English
Author(s) Dupraz Valentin1, Coquille Nathalie1, 2, 3, 4, Menard Dominique1, Sussarellu RossanaORCID1, Haugarreau Larissa1, Stachowski-Haberkorn SabineORCID1
Affiliation(s) 1 : IFREMER, Lab Ecotoxicol, Rue Ile Yeu,BP 21105, F-44311 Nantes 03, France.
2 : Ctr Bordeaux, UR EABX, Irstea, 50 Ave Verdun, F-33612 Cestas, France.
3 : Univ Bordeaux, Lab Phys Tox Chim Environm, UMR 5805, EPOC, 351 Cours Liberat,CS 10004, F-33405 Talence, France.
4 : CNRS, UMR 5805, EPOC, Lab Phys Tox Chim Environm, 351 Cours Liberat,CS 10004, F-33405 Talence, France.
Source Chemosphere (0045-6535) (Pergamon-elsevier Science Ltd), 2016-05 , Vol. 151 , P. 241-252
DOI 10.1016/j.chemosphere.2016.02.073
WOS© Times Cited 14
Keyword(s) Microalgae, Herbicides, Antifouling, Irgarol, Diuron, Diuron resistance
Abstract A wild strain of Chaetoceros calcitrans and wild and diuron-resistant strains of Tetraselmis suecica, were exposed to the PSII inhibitor herbicides diuron and irgarol, individually and in mixtures. The effects of three concentrations of diuron and irgarol and four binary mixtures were evaluated on doubling time, relative reactive oxygen species and lipid content by flow cytometry, and on photosynthetic efficiency by pulse amplitude modulated fluorescence.

In both wild strains, significant effects were observed for each molecule at the highest concentration tested: at irgarol 0.5 μg L−1, C. calcitrans was shown to be more sensitive than T. suecica (+52% and +19% in doubling time, respectively), whereas at diuron 5 μg L−1, T. suecica was more affected (+125% in doubling time) than C. calcitrans (+21%). Overall, irgarol had a higher toxicity at a lower concentration than diuron (no effect at diuron 0.5 μg L−1) for both wild strains. The strongest mixture (irgarol 0.5 μg L−1 + diuron 5 μg L−1) increased doubling time by 356% for T. suecica, thus showing amplified effects when the two compounds were mixed.

Sequencing of the diuron-resistant strain demonstrated a single mutation in the psbA gene coding sequence. Although resistance of this strain to diuron was confirmed with no effect at the highest diuron concentration, no resistance to irgarol was shown. In addition, the mutant strain exposed to the strongest mixture showed a 3.5-fold increase in doubling time compared with irgarol alone, thereby supporting the hypothesis of a biochemical interaction between these two compounds.
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Dupraz Valentin, Coquille Nathalie, Menard Dominique, Sussarellu Rossana, Haugarreau Larissa, Stachowski-Haberkorn Sabine (2016). Microalgal sensitivity varies between a diuron-resistant strain and two wild strains when exposed to diuron and irgarol, alone and in mixtures. Chemosphere, 151, 241-252. Publisher's official version : https://doi.org/10.1016/j.chemosphere.2016.02.073 , Open Access version : https://archimer.ifremer.fr/doc/00319/43047/