FN Archimer Export Format
PT J
TI Microalgal sensitivity varies between a diuron-resistant strain and two wild strains when exposed to diuron and irgarol, alone and in mixtures
BT 
AF DUPRAZ, Valentin
   COQUILLE, Nathalie
   MENARD, Dominique
   SUSSARELLU, Rossana
   HAUGARREAU, Larissa
   STACHOWSKI-HABERKORN, Sabine
AS 1:1;2:1,2,3,4;3:1;4:1;5:1;6:1;
FF 1:;2:;3:PDG-RBE-BE-LBEX;4:PDG-RBE-BE-LBEX;5:PDG-RBE-BE-LBEX;6:PDG-RBE-BE-LBEX;
C1 IFREMER, Lab Ecotoxicol, Rue Ile Yeu,BP 21105, F-44311 Nantes 03, France.
   Ctr Bordeaux, UR EABX, Irstea, 50 Ave Verdun, F-33612 Cestas, France.
   Univ Bordeaux, Lab Phys Tox Chim Environm, UMR 5805, EPOC, 351 Cours Liberat,CS 10004, F-33405 Talence, France.
   CNRS, UMR 5805, EPOC, Lab Phys Tox Chim Environm, 351 Cours Liberat,CS 10004, F-33405 Talence, France.
C2 IFREMER, FRANCE
   IRSTEA, FRANCE
   UNIV BORDEAUX, FRANCE
   CNRS, FRANCE
SI NANTES
SE PDG-RBE-BE-LBEX
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-univ-france
IF 4.208
TC 16
UR https://archimer.ifremer.fr/doc/00319/43047/42658.pdf
LA English
DT Article
DE ;Microalgae;Herbicides;Antifouling;Irgarol;Diuron;Diuron resistance
AB A wild strain of Chaetoceros calcitrans and wild and diuron-resistant strains of Tetraselmis suecica, were exposed to the PSII inhibitor herbicides diuron and irgarol, individually and in mixtures. The effects of three concentrations of diuron and irgarol and four binary mixtures were evaluated on doubling time, relative reactive oxygen species and lipid content by flow cytometry, and on photosynthetic efficiency by pulse amplitude modulated fluorescence.  In both wild strains, significant effects were observed for each molecule at the highest concentration tested: at irgarol 0.5 μg L−1, C. calcitrans was shown to be more sensitive than T. suecica (+52% and +19% in doubling time, respectively), whereas at diuron 5 μg L−1, T. suecica was more affected (+125% in doubling time) than C. calcitrans (+21%). Overall, irgarol had a higher toxicity at a lower concentration than diuron (no effect at diuron 0.5 μg L−1) for both wild strains. The strongest mixture (irgarol 0.5 μg L−1 + diuron 5 μg L−1) increased doubling time by 356% for T. suecica, thus showing amplified effects when the two compounds were mixed.  Sequencing of the diuron-resistant strain demonstrated a single mutation in the psbA gene coding sequence. Although resistance of this strain to diuron was confirmed with no effect at the highest diuron concentration, no resistance to irgarol was shown. In addition, the mutant strain exposed to the strongest mixture showed a 3.5-fold increase in doubling time compared with irgarol alone, thereby supporting the hypothesis of a biochemical interaction between these two compounds.
PY 2016
PD MAY
SO Chemosphere
SN 0045-6535
PU Pergamon-elsevier Science Ltd
VL 151
UT 000374071000030
BP 241
EP 252
DI 10.1016/j.chemosphere.2016.02.073
ID 43047
ER

EF