Development of an ecotoxicological protocol for the deep-sea fauna using the hydrothermal vent shrimp Rimicaris exoculata

Type Article
Date 2016-06
Language English
Author(s) Auguste M.1, Mestre N. C.1, Rocha T. L.1, Cardoso C.1, Cueff-Gauchard Valerie2, 3, 4, Le Bloa Simon2, 3, 4, Cambon-Bonavita Marie-Anne2, 3, 4, Shillito B.5, Zbinden M.5, Ravaux J.5, Bebianno M. J.1
Affiliation(s) 1 : Univ Algarve, CIMA, Fac Sci & Technol, Campus Gambelas, P-8005139 Faro, Portugal.
2 : UBO, Ifremer Ctr Bretagne, REM EEP Lab Microbiol Environm Extremes, UMR 6197,Ifremer,CNRS,ZI Pointe Diable, CS10070, F-29280 Plouzane, France.
3 : UBO, UMR 6197, IFREMER, CNRS, IUEM Rue Dumont dUrville, F-29280 Plouzane, France.
4 : UBO, IFREMER, CNRS, UMR 6197, IUEM Rue Dumont dUrville, F-29280 Plouzane, France.
5 : Univ Paris 04, Univ Paris 06, UMR CNRS 7208, MNHN IRD UCN UA 207,Biol Organismes & Ecosyst Aqu, 7 Quai St Bernard, F-75252 Paris 5, France.
Source Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2016-06 , Vol. 175 , P. 277-285
DOI 10.1016/j.aquatox.2016.03.024
WOS© Times Cited 20
Keyword(s) Deep-sea mining, Hydrothermal vents, Biomarkers, Ipocamp, Copper, TAG
Abstract In light of deep-sea mining industry development, particularly interested in massive-sulphide deposits enriched in metals with high commercial value, efforts are increasing to better understand potential environmental impacts to local fauna. The aim of this study was to assess the natural background levels of biomarkers in the hydrothermal vent shrimp Rimicaris exoculata and their responses to copper exposure at in situ pressure (30 MPa) as well as the effects of depressurization and pressurization of the high-pressure aquarium IPOCAMP. R. exoculata were collected from the chimney walls of the hydrothermal vent site TAG (Mid Atlantic Ridge) at 3630 m depth during the BICOSE cruise in 2014. Tissue metal accumulation was quantified in different tissues (gills, hepatopancreas and muscle) and a battery of biomarkers was measured: metal exposure (metallothioneins), oxidative stress (catalase, superoxide dismutase, glutathione-S-transferase and glutathione peroxidase) and oxidative damage (lipid peroxidation). Data show a higher concentration of Cu in the hepatopancreas and a slight increase in the gills after incubations (for both exposed groups). Significant induction of metallothioneins was observed in the gills of shrimps exposed to 4 μM of Cu compared to the control group. Moreover, activities of enzymes were detected for the in situ group, showing a background protection against metal toxicity. Results suggest that the proposed method, including a physiologically critical step of pressurizing and depressurizing the test chamber to enable the seawater exchange during exposure to contaminants, is not affecting metal accumulation and biomarkers response and may prove a useful method to assess toxicity of contaminants in deep-sea species.
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Auguste M., Mestre N. C., Rocha T. L., Cardoso C., Cueff-Gauchard Valerie, Le Bloa Simon, Cambon-Bonavita Marie-Anne, Shillito B., Zbinden M., Ravaux J., Bebianno M. J. (2016). Development of an ecotoxicological protocol for the deep-sea fauna using the hydrothermal vent shrimp Rimicaris exoculata. Aquatic Toxicology, 175, 277-285. Publisher's official version : https://doi.org/10.1016/j.aquatox.2016.03.024 , Open Access version : https://archimer.ifremer.fr/doc/00323/43465/