TY - JOUR T1 - Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix A1 - Landreau,M. A1 - Duthoit,Frederique A1 - Claeys-Bruno,M. A1 - Vandenabeele-Trambouze,O. A1 - Aubry,T. A1 - Godfroy,Anne A1 - Le Blay,Gwenaelle AD - Univ Bretagne Occidentale, IUEM, Lab Microbiol Environm Extremes, UMR6197, Technopole Brest Iroise, Plouzane, France. AD - IFREMER, Lab Microbiol Environm Extremes, UMR6197, Technopole Brest Iroise, Plouzane, France. AD - CNRS, Lab Microbiol Environm Extremes, UMR6197, Technopole Brest Iroise, Plouzane, France. AD - Aix Marseille Univ, LISA EA4672, Marseille, France. AD - UFR Sci & Tech, LIMATB, Lab Ingn Mat Bretagne, Equipe Rheol, Brest, France. UR - https://doi.org/10.1111/jam.13118 DO - 10.1111/jam.13118 KW - (hyper)thermophilic marine micro-organisms KW - anaerobiosis KW - entrapment KW - gellan KW - immobilization KW - xanthan N2 - Aims The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. Methods and Results The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (<0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect on cell viability and a good growth within and outside the beads. Conclusion This suggests that entrapment in a gellan–xanthan matrix could be employed for the culture of anaerobic (hyper)thermophilic marine micro-organisms. Significance and Impact of the Study (Hyper)thermophilic marine micro-organisms possess a high biotechnological potential. Generally microbial cells are grown as free-cell cultures. The use of immobilized cells may offer several advantages such as protection against phage attack, high cell biomass and better production rate of desired metabolites. Y1 - 2016/06 PB - Wiley-blackwell JF - Journal Of Applied Microbiology SN - 1364-5072 VL - 120 IS - 6 SP - 1531 EP - 1541 ID - 44352 ER -