FN Archimer Export Format
PT J
TI Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix
BT 
AF LANDREAU, M.
   DUTHOIT, Frederique
   CLAEYS-BRUNO, M.
   VANDENABEELE-TRAMBOUZE, O.
   AUBRY, T.
   GODFROY, Anne
   LE BLAY, Gwenaelle
AS 1:1,2,3;2:1,2,3;3:4;4:1,2,3;5:5;6:1,2,3;7:1,2,3;
FF 1:;2:;3:;4:;5:;6:PDG-REM-EEP-LMEE;7:;
C1 Univ Bretagne Occidentale, IUEM, Lab Microbiol Environm Extremes, UMR6197, Technopole Brest Iroise, Plouzane, France.
   IFREMER, Lab Microbiol Environm Extremes, UMR6197, Technopole Brest Iroise, Plouzane, France.
   CNRS, Lab Microbiol Environm Extremes, UMR6197, Technopole Brest Iroise, Plouzane, France.
   Aix Marseille Univ, LISA EA4672, Marseille, France.
   UFR Sci & Tech, LIMATB, Lab Ingn Mat Bretagne, Equipe Rheol, Brest, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
   CNRS, FRANCE
   UNIV AIX MARSEILLE, FRANCE
   UBO, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
UM BEEP-LM2E
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.099
TC 6
UR https://archimer.ifremer.fr/doc/00332/44352/44000.pdf
LA English
DT Article
DE ;(hyper)thermophilic marine micro-organisms;anaerobiosis;entrapment;gellan;immobilization;xanthan
AB Aims  The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. Methods and Results  The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (<0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect on cell viability and a good growth within and outside the beads. Conclusion  This suggests that entrapment in a gellan–xanthan matrix could be employed for the culture of anaerobic (hyper)thermophilic marine micro-organisms. Significance and Impact of the Study  (Hyper)thermophilic marine micro-organisms possess a high biotechnological potential. Generally microbial cells are grown as free-cell cultures. The use of immobilized cells may offer several advantages such as protection against phage attack, high cell biomass and better production rate of desired metabolites.
PY 2016
PD JUL
SO Journal Of Applied Microbiology
SN 1364-5072
PU Wiley-blackwell
VL 120
IS 6
UT 000379534800008
BP 1531
EP 1541
DI 10.1111/jam.13118
ID 44352
ER

EF