FN Archimer Export Format
PT J
TI First study in cryopreserved Crassostrea angulata sperm
BT 
AF RIESCO, Marta F.
   FELIX, Francisca
   MATIAS, Domitilia
   JOAQUIM, Sandra
   SUQUET, Marc
   CABRITA, Elsa
AS 1:1;2:1;3:2;4:2;5:3;6:1;
FF 1:;2:;3:;4:;5:PDG-RBE-PFOM-PI;6:;
C1 Univ Algarve, CCMAR, Campus Gambelas, P-8005139 Faro, Portugal.
   IP, IPMA, Av 5 Outubro S-N, P-8700305 Olhao, Portugal.
   IFREMER, UMR LEMAR 6539, Dept PFOM, Lab ARN,Stn Expt Argenton, F-29840 Presquile Vivier, Argenton, France.
C2 UNIV ALGARVE, PORTUGAL
   IPMA, PORTUGAL
   IFREMER, FRANCE
SI ARGENTON
SE PDG-RBE-PFOM-PI
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 2.564
TC 11
UR https://archimer.ifremer.fr/doc/00333/44432/44098.pdf
LA English
DT Article
DE ;Crassostrea angulata;Sperm;Cryopreservation;Motility;Viability;Fertilization
AB Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry. However, inadequate management of this industry and pathology occurrences resulted in a significant decrease in natural populations. For this reason, in this work a successful sperm cryopreservation protocol for this important species has been developed for the first time. Different internal cryoprotectants (DMSO, ethylene glycol, polyethylene glycol and methanol) at several concentrations (5, 10, 20%), containers (straws vs cryovials) and freezing rates (slow and fast rates) were tested. Cryoprotectant toxicity tests corroborated that this assay did not take into account the following steps of cryopreservation protocol as sperm agglutination. A fast freezing rate of cells diluted in10% DMSO and the use of straws as containers were the best cryopreservation conditions for Portuguese oyster sperm. Finally, fertilization assays confirmed the efficiency of the cryopreservation protocol in oyster sperm. These results demonstrated that different susceptibilities have been detected concerning sperm cryopreservation depending on oyster species or genetic material composition.
PY 2017
PD MAY
SO General And Comparative Endocrinology
SN 0016-6480
PU Academic Press Inc Elsevier Science
VL 245
UT 000399063700015
BP 108
EP 115
DI 10.1016/j.ygcen.2016.05.003
ID 44432
ER

EF