FN Archimer Export Format PT J TI Anti-Metastatic Properties of a Marine Bacterial Exopolysaccharide-Based Derivative Designed to Mimic Glycosaminoglycans BT AF HEYMANN, Dominique RUIZ-VELASCO, Carmen CHESNEAU, Julie RATISKOL, Jacqueline SINQUIN, Corinne COLLIEC-JOUAULT, Sylvia AS 1:1;2:1;3:1;4:2;5:2;6:2; FF 1:;2:;3:;4:PDG-RBE-BRM-LEMMMB;5:PDG-RBE-BRM-LEMMMB;6:PDG-RBE-BRM-LEMMMB; C1 INSERM, UMR957, Lab Physiopathol Resorpt Osseuse & Therapie Tumeu, Equipe Ligue Canc 2012, F-44035 Nantes, France. IFREMER, Lab Ecosyst Microbiens & Mol Marines Biotechnol E, BP21105, F-44311 Nantes, France. C2 INSERM, FRANCE IFREMER, FRANCE SI NANTES SE PDG-RBE-BRM-LEMMMB IN WOS Ifremer jusqu'en 2018 DOAJ copubli-france IF 2.861 TC 22 UR https://archimer.ifremer.fr/doc/00333/44442/44112.pdf https://archimer.ifremer.fr/doc/00333/44442/44113.pdf LA English DT Article DE ;exopolysaccharides;glycosaminoglycan;heparin-like;derivatives;sulfation;bone metabolism;bone remodeling;lung mestatases;osteosarcoma AB Osteosarcoma is the most frequent malignant primary bone tumor characterized by a high potency to form lung metastases. In this study, the effect of three oversulfated low molecular weight marine bacterial exopolysaccharides (OS-EPS) with different molecular weights (4, 8 and 15 kDa) were first evaluated in vitro on human and murine osteosarcoma cell lines. Different biological activities were studied: cell proliferation, cell adhesion and migration, matrix metalloproteinase expression. This in vitro study showed that only the OS-EPS 15 kDa derivative could inhibit the invasiveness of osteosarcoma cells with an inhibition rate close to 90%. Moreover, this derivative was potent to inhibit both migration and invasiveness of osteosarcoma cell lines; had no significant effect on their cell cycle; and increased slightly the expression of MMP-9, and more highly the expression of its physiological specific tissue inhibitor TIMP-1. Then, the in vivo experiments showed that the OS-EPS 15 kDa derivative had no effect on the primary osteosarcoma tumor induced by osteosarcoma cell lines but was very efficient to inhibit the establishment of lung metastases in vivo. These results can help to better understand the mechanisms of GAGs and GAG-like derivatives in the biology of the tumor cells and their interactions with the bone environment to develop new therapeutic strategies. PY 2016 PD MAR SO Molecules SN 1420-3049 PU Mdpi Ag VL 21 IS 3 UT 000373802200064 DI 10.3390/molecules21030309 ID 44442 ER EF