FN Archimer Export Format PT J TI Contrasting effects of historical contingency on phenotypic and genomic trajectories during a two-step evolution experiment with bacteria BT AF PLUCAIN, Jessica SUAU, Antonia CRUVEILLER, Stephane MEDIGUE, Claudine SCHNEIDER, Dominique LE GAC, Mickael AS 1:1,2;2:1,2,3;3:4,5,6;4:4,5,6;5:1,2;6:1,2,7; FF 1:;2:;3:;4:;5:;6:PDG-ODE-DYNECO-PELAGOS; C1 Univ Grenoble Alpes, Lab Technol Ingn Med & Complex Informat Math & Ap, F-38000 Grenoble, France. CNRS, TIMC IMAG, F-38000 Grenoble, France. Conservatoire Natl Arts & Metiers, Paris, France. CEA, Direct Sci Vivant, Inst Genom, Genoscope, Evry, France. CNRS UMR8030, Evry, France. Lab Anal Bioinformat Genom & Metab, Evry, France. IFREMER, DYNECO Pelagos, F-29280 Plouzane, France. C2 UNIV GRENOBLE ALPES, FRANCE CNRS, FRANCE CNAM, FRANCE CEA, FRANCE CNRS, FRANCE GENOSCOPE, FRANCE IFREMER, FRANCE SI BREST SE PDG-ODE-DYNECO-PELAGOS IN WOS Ifremer jusqu'en 2018 DOAJ copubli-france copubli-univ-france IF 3.221 TC 12 UR https://archimer.ifremer.fr/doc/00333/44447/44118.pdf https://archimer.ifremer.fr/doc/00333/44447/44119.pdf LA English DT Article DE ;Experimental evolution;Escherichia coli;Adaptation;Historical contingency;Epistasis AB Background The impact of historical contingency, i.e. the past evolutionary history of a population, on further adaptation is mostly unknown at both the phenotypic and genomic levels. We addressed this question using a two-step evolution experiment. First, replicate populations of Escherichia coli were propagated in four different environmental conditions for 1000 generations. Then, all replicate populations were transferred and propagated for further 1000 generations to a single new environment. Results Using this two-step experimental evolution strategy, we investigated, at both the phenotypic and genomic levels, whether and how adaptation in the initial historical environments impacted evolutionary trajectories in a new environment. We showed that both the growth rate and fitness of the evolved populations obtained after the second step of evolution were contingent upon past evolutionary history. In contrast however, the genes that were modified during the second step of evolution were independent from the previous history of the populations. Conclusions Our work suggests that historical contingency affects phenotypic adaptation to a new environment. This was however not reflected at the genomic level implying complex relationships between environmental factors and the genotype-to-phenotype map. PY 2016 PD APR SO Bmc Evolutionary Biology SN 1471-2148 PU Biomed Central Ltd VL 16 UT 000374742400001 DI 10.1186/s12862-016-0662-8 ID 44447 ER EF