FN Archimer Export Format
PT J
TI Fish nodavirus lytic cycle and semipermissive expression in mammalian and fish cell cultures
BT 
AF DELSERT, Claude
   MORIN, Nathalie
   COMPS, Michel
AS 1:1;2:2;3:3;
FF 1:;2:;3:;
C1 IFREMER, STN MEDITERRANEENNE ENVIRONM LITTORAL, 1 QUAI DAURADE, F-34200 SETE, FRANCE.
   CNRS, CRBM, F-34033 MONTPELLIER, FRANCE.
   IFREMER, F-34240 PALAVAS LES FLOTS, FRANCE.
C2 IFREMER, FRANCE
   CNRS, FRANCE
   IFREMER, FRANCE
IN WOS Ifremer jusqu'en 2018
IF 5.93
TC 46
UR https://archimer.ifremer.fr/doc/00336/44697/44983.pdf
LA English
DT Article
AB In this study, Dicentrarchus labrax encephalitis virus (DIEV), which causes sea bass encephalitis, was propagated in cell culture, thus allowing study of its lytic cycle, DIEV infection of mammalian and fish cells induced different patterns of expression of capsid proteins, which were assembled as virus-like particles, accumulating in the cytoplasm either as diffuse masses or in vesicles, as shown by electron microscopy, These particles correspond to virions, as shown by their ability to induce Secondary infection, Fish cells proved to be more permissive for DIEV than mammalian cells, although virus yield remained low, RNA analysis of infected sea bass cells revealed DIEV RNA3, in addition to genomic RNA1 and RNA2, and the presence of the RNA;! minus strand, thus demonstrating the replication of the DIEV genome, In addition, DIEV RNA-dependent RNA polymerase was associated with mature virions even after purification by a CsCl gradient, but it was dissociated when capsids were destabilized, In addition to providing more information about the relatedness of DIEV to the members of the family Nodaviridae, this study shows that fish nodaviruses may not be able to infect as wide a variety of cells as insect nodaviruses can.
PY 1997
PD JUN
SO Journal Of Virology
SN 0022-538X
PU Amer Soc Microbiology
VL 71
IS 7
UT A1997XD82900099
BP 5673
EP 5677
ID 44697
ER

EF