Molecular epidemiological survey of rotaviruses in sewage by reverse transcriptase seminested PCR and restriction fragment length polymorphism assay
|Author(s)||Dubois E1, Le Guyader Soizick1, Haugarreau Larissa1, Kopecka H2, Cormier M3, Pommepuy Monique1|
|Affiliation(s)||1 : IFREMER,MICROBIOL LAB,BP 21105,F-44311 NANTES 03,FRANCE.
2 : INST PASTEUR,UNITE VIROL MOL,PARIS,FRANCE.
3 : UER MED & PHARMACEUT,LAB MICROBIOL PHARMACEUT,RENNES,FRANCE.
|Source||Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 1997-05 , Vol. 63 , N. 5 , P. 1794-1800|
|WOS© Times Cited||47|
|Abstract||Rotavirus double-stranded RNA was detected directly in sewage treatment plant samples over a 1-year period by reverse transcription followed by PCR amplification of the VP7 gene and Southern blot hybridization. The presence of naturally occurring rotaviruses was demonstrated in 42% of raw sewage samples and in 67% of treated effluent samples, Amplified viral sequences were analyzed bg restriction enzymes. Ten different restriction profiles were characterized, most of which were found in treated effluent samples. A mixture of restriction profiles was observed in 75% of contaminated effluent samples, The profiles were compared with those obtained from human rotavirus isolates involved in infections in children from the same area (six different profiles were detected), Five identical viral sequences were detected in both environmental and clinical samples, Restriction profiles sere also compared io profiles from known genomic sequences of human and animal viruses. Both human and animal origins of rotavirus contamination of water seemed likely.|