FN Archimer Export Format
PT J
TI Molecular epidemiological survey of rotaviruses in sewage by reverse transcriptase seminested PCR and restriction fragment length polymorphism assay
BT 
AF DUBOIS, E
   LE GUYADER, Soizick
   HAUGARREAU, Larissa
   KOPECKA, H
   CORMIER, M
   POMMEPUY, Monique
AS 1:1;2:1;3:1;4:2;5:3;6:1;
FF 1:;2:;3:;4:;5:;6:;
C1 IFREMER,MICROBIOL LAB,BP 21105,F-44311 NANTES 03,FRANCE.
   INST PASTEUR,UNITE VIROL MOL,PARIS,FRANCE.
   UER MED & PHARMACEUT,LAB MICROBIOL PHARMACEUT,RENNES,FRANCE.
C2 IFREMER, FRANCE
   INST PASTEUR, FRANCE
   UNIV RENNES, FRANCE
IN WOS Ifremer jusqu'en 2018
IF 3.389
TC 47
UR https://archimer.ifremer.fr/doc/00336/44720/44851.pdf
LA English
DT Article
AB Rotavirus double-stranded RNA was detected directly in sewage treatment plant samples over a 1-year period by reverse transcription followed by PCR amplification of the VP7 gene and Southern blot hybridization. The presence of naturally occurring rotaviruses was demonstrated in 42% of raw sewage samples and in 67% of treated effluent samples, Amplified viral sequences were analyzed bg restriction enzymes. Ten different restriction profiles were characterized, most of which were found in treated effluent samples. A mixture of restriction profiles was observed in 75% of contaminated effluent samples, The profiles were compared with those obtained from human rotavirus isolates involved in infections in children from the same area (six different profiles were detected), Five identical viral sequences were detected in both environmental and clinical samples, Restriction profiles sere also compared io profiles from known genomic sequences of human and animal viruses. Both human and animal origins of rotavirus contamination of water seemed likely.
PY 1997
PD MAY
SO Applied And Environmental Microbiology
SN 0099-2240
PU Amer Soc Microbiology
VL 63
IS 5
UT A1997WX36100025
BP 1794
EP 1800
ID 44720
ER

EF