FN Archimer Export Format PT J TI Effect of electrolysis treatment on the biomineralization capacities of pearl oyster Pinctada margaritifera juveniles BT AF LATCHERE, Oihana FIEVET, Julie LO, Cedrik SCHNEIDER, Denis DIEU, Stephanie CABRAL, Philippe BELLIARD, Corinne KY, Chin-Long GUEGUEN, Yannick SAULNIER, Denis AS 1:1;2:1;3:2;4:3;5:3;6:4;7:1;8:1;9:1,5;10:1; FF 1:PDG-RBE-RMPF;2:PDG-RBE-RMPF;3:;4:;5:;6:;7:PDG-RBE-RMPF;8:PDG-RBE-RMPF;9:PDG-RBE-IHPE;10:PDG-RBE-RMPF; C1 UPF ILM IRD, UMR EIO 241, IFREMER, Labex Corail, BP 7004, Taravao 98719, Tahiti, Fr Polynesia. Direct Ressources Marines & Minieres, Tahiti, Fr Polynesia. Espace Bleu, Bora Bora, Fr Polynesia. Gauguins Pearl Farm, Rangiroa, Fr Polynesia. Univ Montpellier, CNRS, UPVD, Ifremer,UMR IPHE 5244, CC 80, F-34095 Montpellier, France. C2 IFREMER, FRANCE DIRECT RESSOURCES MARINES & MINIERES, FRANCE ESPACE BLEU, FRANCE GAUGUINS PEARL FARM, FRANCE IFREMER, FRANCE SI TAHITI MONTPELLIER SE PDG-RBE-RMPF PDG-RBE-IHPE UM IHPE EIO IN WOS Ifremer jusqu'en 2018 copubli-france IF 2.176 TC 3 UR https://archimer.ifremer.fr/doc/00343/45410/44924.pdf LA English DT Article DE ;Pearl oyster;Pinctada margaritifera;Electrolysis;Biomineralization;Gene expression AB The present study investigated the effect of electrolysis on the biomineralization capacities of juveniles of the mollusk Pinctada margaritifera for the first time. Size-selected individuals from two groups, “Medium” and “Large”, from a multi-parental family produced in a hatchery system were subjected to electrolysis under a low voltage current over a nine-week experimental period. The growth of the juveniles was individually monitored and assessed weekly by wet weight and shell height measurements. At the end of the experiment, mantle tissue was sampled for biomineralization-related gene expression analysis. Electrolysis significantly increased pearl oyster growth in terms of shell height and wet weight for Large juveniles from the 5th and the 2nd week, respectively, until the end of the experiment. However, differences were only significant for Medium individuals from the 7th week for shell height and from the 9th week for wet weight. Furthermore, transcriptional analysis of six known biomineralization genes coding for shell matrix proteins of calcitic prisms and/or nacreous shell structures revealed that five were significantly overexpressed in the mantle mineralizing tissue under electrolysis: three in common between the two size class groups and two that were expressed exclusively in one or the other group. Finally, we found no statistical difference of the shell thickness ratio between individuals undergoing electrolysis and control conditions. Taken together, our results indicate, for the first time in a calcifying marine organism, that electrolysis influences molecular mechanisms involved in biomineralization and may stimulate some parameters of pearl oyster growth rate. PY 2016 PD DEC SO Estuarine Coastal And Shelf Science SN 0272-7714 PU Academic Press Ltd- Elsevier Science Ltd VL 182 IS Part.B UT 000390728100003 BP 235 EP 242 DI 10.1016/j.ecss.2016.06.014 ID 45410 ER EF