Evidence of the bioaccumulation of ciguatoxins in giant clams (Tridacna maxima) exposed to Gambierdiscus spp. cells

Type Article
Date 2016-07
Language English
Author(s) Roue Melanie1, Darius Helene Taiana2, Picot Sandy1, 2, Ung Andre2, Viallon Jerome2, Gaertner-Mazouni Nabila3, Sibat ManoellaORCID4, Amzil ZouherORCID4, Chinain Mireille2
Affiliation(s) 1 : IRD, UMR EIO 241, POB 529, F-98713 Papeete, Tahiti, Fr Polynesia.
2 : ILM, Lab Tox Microalgae UMR EIO 241, POB 30, F-98713 Papeete, Tahiti, Fr Polynesia.
3 : UPF, UMR EIO 241, POB 6570, F-98702 Faaa, Tahiti, France.
4 : IFREMER, Phycotoxins Lab, POB 21105, F-44311 Nantes, France.
Source Harmful Algae (1568-9883) (Elsevier Science Bv), 2016-07 , Vol. 57 , N. Part. A , P. 78-87
DOI 10.1016/j.hal.2016.05.007
WOS© Times Cited 42
Keyword(s) Giant clams, Ex situ contamination, Ciguatoxins, Gambierdiscus polynesiensis, LC-MS/MS, Neuroblastoma cell-based assay
Abstract Ciguatera Fish Poisoning (CFP) is a foodborne disease classically related to the consumption of tropical coral reef fishes contaminated with ciguatoxins (CTXs), neurotoxins produced by dinoflagellates of the Gambierdiscus genus. Severe atypical ciguatera-like incidents involving giant clams, a marine resource highly consumed in the South Pacific, are also frequently reported in many Pacific Islands Countries and Territories. The present study was designed to assess the ability of giant clams to accumulate CTXs in their tissues and highlight the potential health risks associated with their consumption. Since giant clams are likely to be exposed to both free-swimming Gambierdiscus cells and dissolved CTXs in natural environment, ex situ contamination experiments were conducted as follows: giant clams were exposed to live or lyzed cells of TB92, a highly toxic strain of G. polynesiensis containing 5.83 ± 0.85 pg P-CTX-3C equiv. cell−1vs. HIT0, a weakly toxic strain of G. toxicus containing only (2.05 ± 1.16) × 10−3 pg P-CTX-3C equiv. cell−1, administered over a 48 h period at a concentration of 150 cells mL−1. The presence of CTXs in giant clams tissues was further assessed using the mouse neuroblastoma cell-based assay (CBA-N2a). Results showed that giant clams exposed to either lyzed or live cells of TB92 were able to bioaccumulate CTXs at concentrations well above the safety limit recommended for human consumption, i.e. 3.28 ± 1.37 and 2.92 ± 1.03 ng P-CTX-3C equiv. g−1 flesh (wet weight), respectively, which represented approximately 3% of the total toxin load administered to the animals. In contrast, giant clams exposed to live or lyzed cells of HIT0 were found to be free of toxins, suggesting that in the nature, the risk of contamination of these bivalves is established only in the presence of highly toxic blooms of Gambierdiscus. Liquid chromatography–mass spectrometry (LC–MS/MS) analyses confirmed CBA-N2a results and also revealed that P-CTX-3B was the major CTX congener retained in the tissues of giant clams fed with TB92 cells. To the best of our knowledge, this study is the first to provide evidence of the bioaccumulation of Gambierdiscus CTXs in giant clams and confirms that these bivalve molluscs can actually constitute another pathway in ciguatera poisonings. While most monitoring programs currently focus on fish toxicity, these findings stress the importance of a concomitant surveillance of these marine invertebrates in applicable locations for an accurate assessment of ciguatera risk.
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Roue Melanie, Darius Helene Taiana, Picot Sandy, Ung Andre, Viallon Jerome, Gaertner-Mazouni Nabila, Sibat Manoella, Amzil Zouher, Chinain Mireille (2016). Evidence of the bioaccumulation of ciguatoxins in giant clams (Tridacna maxima) exposed to Gambierdiscus spp. cells. Harmful Algae, 57(Part. A), 78-87. Publisher's official version : https://doi.org/10.1016/j.hal.2016.05.007 , Open Access version : https://archimer.ifremer.fr/doc/00343/45415/