FN Archimer Export Format
PT J
TI Molecular Profiling of Shiga Toxin-Producing Escherichia coli and Enteropathogenic E. coli Strains Isolated from French Coastal Environments
BT 
AF BALIERE, Charlotte
   RINCE, A.
   DELANNOY, S.
   FACH, P.
   GOURMELON, Michele
AS 1:1;2:2;3:3;4:3;5:1;
FF 1:PDG-RBE-SG2M-LSEM;2:;3:;4:;5:PDG-RBE-SG2M-LSEM;
C1 IFREMER, Dept Ressources Biol & Environm, Unite Sante Genet & Microbiol Mollusques, Lab Sante Environm & Microbiol, Plouzane, France.
   Normandie Univ UCBN, U2RM EA4655 Stress Virulence, Caen, France.
   Univ Paris Est, ANSES Food Safety Lab, Platform IdentyPath, Maisons Alfort, France.
C2 IFREMER, FRANCE
   UNIV CAEN NORMANDIE, FRANCE
   UNIV PARIS EST, FRANCE
SI BREST
SE PDG-RBE-SG2M-LSEM
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.807
TC 22
UR https://archimer.ifremer.fr/doc/00344/45514/45076.pdf
LA English
DT Article
AB Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) strains may be responsible for food-borne infections in humans. Twenty-eight STEC and 75 EPEC strains previously isolated from French shellfish-harvesting areas and their watersheds and belonging to 68 distinguishable serotypes were characterized in this study. High-throughput real-time PCR was used to search for the presence of 75 E. coli virulence-associated gene targets, and genes encoding Shiga toxin (stx) and intimin (eae) were subtyped using PCR tests and DNA sequencing, respectively. The results showed a high level of diversity between strains, with 17 unique virulence gene profiles for STEC and 56 for EPEC. Seven STEC and 15 EPEC strains were found to display a large number or a particular combination of genetic markers of virulence and the presence of stx and/or eae variants, suggesting their potential pathogenicity for humans. Among these, an O26:H11 stx1a eae-β1 strain was associated with a large number of virulence-associated genes (n = 47), including genes carried on the locus of enterocyte effacement (LEE) or other pathogenicity islands, such as OI-122, OI-71, OI-43/48, OI-50, OI-57, and the high-pathogenicity island (HPI). One O91:H21 STEC strain containing 4 stx variants (stx1a, stx2a, stx2c, and stx2d) was found to possess genes associated with pathogenicity islands OI-122, OI-43/48, and OI-15. Among EPEC strains harboring a large number of virulence genes (n, 34 to 50), eight belonged to serotype O26:H11, O103:H2, O103:H25, O145:H28, O157:H7, or O153:H2.
PY 2016
PD JUN
SO Applied And Environmental Microbiology
SN 0099-2240
PU Amer Soc Microbiology
VL 82
IS 13
UT 000378048800024
BP 3913
EP 3927
DI 10.1128/AEM.00271-16
ID 45514
ER

EF