FN Archimer Export Format
PT J
TI Optimization of growth and bacteriocin activity of the food bioprotective Carnobacterium divergens V41 in an animal origin protein free medium
BT 
AF BRILLET-VIEL, Anne
   PILET, Marie-France
   COURCOUX, Philippe
   PREVOST, Hervé
   LEROI, Francoise
AS 1:1;2:1;3:2;4:1;5:3;
FF 1:;2:;3:;4:;5:PDG-RBE-BRM-LEMMMB;
C1 Univ Bretagne Loire, ONIRIS, UMR 1014, SECALIM, Nantes, France
   Univ Bretagne Loire, ONIRIS, USC Inst Natl Rech Agron Sensometrie & Chimiometi, Nantes, France
   IFREMER, Lab Ecosyst Microbiens & Mol Marines Biotechnol, Nantes, France
C2 ONIRIS, FRANCE
   ONIRIS, FRANCE
   IFREMER, FRANCE
SI NANTES
SE PDG-RBE-BRM-LEMMMB
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
IF 5.247
TC 12
UR https://archimer.ifremer.fr/doc/00345/45615/45236.pdf
LA English
DT Article
DE ;optimization;Carnobacterium;bacteriocin;experimental design;bioreactor;Growth;Cold-smoked salmon
AB Optimization of Carnobacterium divergens V41 growth and bacteriocin activity in a culture medium deprived of animal protein, needs for food bioprotection, was performed by using a statistical approach. In a screening experiment, twelve factors (pH, temperature, carbohydrates, NaCl, yeast extract, soy peptone, sodium acetate, ammonium citrate, magnesium sulphate, manganese sulphate, ascorbic acid and thiamine) were tested for their influence on the maximal growth and bacteriocin activity using a two-level incomplete factorial design with 192 experiments performed in microtiter plate wells. Based on results, a basic medium was developed and three variables (pH, temperature and carbohydrates concentration) were selected for a scale-up study in bioreactor. A 23 complete factorial design was performed, allowing the estimation of linear effects of factors and all the first order interactions. The best conditions for the cell production were obtained with a temperature of 15°C and a carbohydrates concentration of 20 g/l whatever the pH (in the range 6.5-8), and the best conditions for bacteriocin activity were obtained at 15°C and pH 6.5 whatever the carbohydrates concentration (in the range 2-20 g/l). The predicted final count of C. divergens V41 and the bacteriocin activity under the optimized conditions (15°C, pH 6.5, 20 g/l carbohydrates) were 2.4 x 1010 CFU/ml and 819200 AU/ml respectively. C. divergens V41 cells cultivated in the optimized conditions were able to grow in cold-smoked salmon and totally inhibited the growth of Listeria monocytogenes (< 50 CFU g-1) during five weeks of vacuum storage at 4° and 8°C.
PY 2016
PD AUG
SO Frontiers in Marine Science
SN 2296-7745
PU Frontiers Media S.A
VL 3
IS 128
UT 000457358000125
DI 10.3389/fmars.2016.00128
ID 45615
ER

EF