FN Archimer Export Format
PT J
TI Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae
BT 
AF GERVAIS, Ophelie
   CHOLLET, Bruno
   RENAULT, Tristan
   ARZUL, Isabelle
AS 1:1;2:1;3:2;4:1;
FF 1:PDG-RBE-SG2M-LGPMM;2:PDG-RBE-SG2M-LGPMM;3:PDG-RBE;4:PDG-RBE-SG2M-LGPMM;
C1 IFREMER, RBE, SG2M, LGPMM,Stn La Tremblade, Ave Mus Loup, F-17390 La Tremblade, France.
   IFREMER, RBE, Ctr Nantes, Rue Ile Yeu, F-44311 Nantes, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI LA TREMBLADE
   NANTES
SE PDG-RBE-SG2M-LGPMM
   PDG-RBE
IN WOS Ifremer jusqu'en 2018
IF 3.148
TC 16
UR https://archimer.ifremer.fr/doc/00346/45692/45312.pdf
LA English
DT Article
DE ;Apoptosis;Host-parasite interactions;Parasite;Oyster;Bonamia ostreae;Ostrea edulis
AB The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses. Whatever the host species was, the parasite was engulfed in hemocytes and induced an increase of apoptotic parameters including intracytoplasmic calcium concentration, mitochondrial membrane potential or phosphatidyl-serine externalization as well as ultrastructural modifications. However, the parasite appears more able to infect flat oyster than cupped oyster hemocytes and the apoptotic response was more important against live than dead parasites in the natural host than in C. gigas. Our results suggest that O. edulis specifically responds to B. ostreae by inducing apoptosis of hemocytes.
PY 2016
PD SEP
SO Fish & Shellfish Immunology
SN 1050-4648
PU Academic Press Ltd- Elsevier Science Ltd
VL 56
UT 000383292200033
BP 322
EP 329
DI 10.1016/j.fsi.2016.07.021
ID 45692
ER

EF