Bioassay battery interlaboratory investigation of emerging contaminants in spiked water extracts – Towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring

Type Article
Date 2016-11
Language English
Author(s) Di Paolohttps://w3.Ifremer.Fr/archimer-Admin/author.Jsp# Carolina1, Ottermanns Richard1, Keiter Steffen1, 2, Ait-Aissa Selim3, Bluhm Kerstin1, Brack Werner4, Breitholtz Magnus5, Buchinger Sebastian6, Carere Mario7, Chalon Carole8, Cousin Xavier9, 10, Dulio Valeria3, Escher Beate I.4, 11, 12, Hamers Timo13, Hilscherova Klara14, Jarque Sergio14, Jonas Adam14, Maillot-Marechal Emmanuelle3, Marneffe Yves8, Mai Thao Nguyen 15, Pandard Pascal3, Schifferli Andrea16, Schulze Tobias4, Seidensticker Sven1, 12, Seiler Thomas-Benjamin1, Tang Janet11, Van Der Oost Ron17, Vermeirssen Etienne16, Zounkova Radka14, Zwart Nick13, Hollert Henner1
Affiliation(s) 1 : Rhein Westfal TH Aachen, Inst Environm Res, Aachen, Germany.
2 : Univ Orebro, Sch Sci & Technol, Man Technol Environm Res Ctr, Orebro, Sweden.
3 : INERIS, Verneuil En Halatte, France.
4 : UFZ Helmholtz Ctr Environm Res, Leipzig, Germany.
5 : Stockholm Univ, Dept Appl Environm Sci ITM, Stockholm, Sweden.
6 : Fed Inst Hydrol, Dept Biochem & Ecotoxicol, Koblenz, Germany.
7 : Italian Inst Hlth, Rome, Italy.
8 : ISSeP Sci Inst Publ Serv, Liege, Wallonia, Belgium.
9 : IFREMER, Lab Ecotoxicol, Lhoumeau, France.
10 : INRA, Lab Physiol & Genet Poissons, Rennes, France.
11 : Univ Queensland, Natl Res Ctr Environm Toxicol Entox, Brisbane, Qld, Australia.
12 : Univ Tubingen, Ctr Appl Geosci, Tubingen, Germany.
13 : Vrije Univ Amsterdam, Inst Environm Studies IVM, Amsterdam, Netherlands.
14 : Masaryk Univ, Res Ctr Tox Cpds Environm RECETOX, Fac Sci, Brno, Czech Republic.
15 : Waterproef Lab, Edam, Netherlands.
16 : Swiss Ctr Appl Ecotoxicol Eawag EPFL, Dubendorf, Switzerland.
17 : WATERNET Inst Urban Water Cycle, Div Technol Res & Engn, Amsterdam, Netherlands.
Source Water Research (0043-1354) (Pergamon-elsevier Science Ltd), 2016-11 , Vol. 104 , P. 473-484
DOI 10.1016/j.watres.2016.08.018
WOS© Times Cited 33
Keyword(s) Triclosan, Acridine, 17 alpha-ethinylestradiol, 3-Nitrobenzanthrone, Organism-level toxicity, Mechanism-specific toxicity
Abstract Bioassays are particularly useful tools to link the chemical and ecological assessments in water quality monitoring. Different methods cover a broad range of toxicity mechanisms in diverse organisms, and account for risks posed by non-target compounds and mixtures. Many tests are already applied in chemical and waste assessments, and stakeholders from the science-police interface have recommended their integration in regulatory water quality monitoring. Still, there is a need to address bioassay suitability to evaluate water samples containing emerging pollutants, which are a current priority in water quality monitoring. The presented interlaboratory study (ILS) verified whether a battery of miniaturized bioassays, conducted in 11 different laboratories following their own protocols, would produce comparable results when applied to evaluate blinded samples consisting of a pristine water extract spiked with four emerging pollutants as single chemicals or mixtures, i.e. triclosan, acridine, 17α-ethinylestradiol (EE2) and 3-nitrobenzanthrone (3-NBA). Assays evaluated effects on aquatic organisms from three different trophic levels (algae, daphnids, zebrafish embryos) and mechanism-specific effects using in vitro estrogenicity (ER-Luc, YES) and mutagenicity (Ames fluctuation) assays. The test battery presented complementary sensitivity and specificity to evaluate the different blinded water extract spikes. Aquatic organisms differed in terms of sensitivity to triclosan (algae > daphnids > FET) and acridine (FET > daphnids > algae) spikes, confirming the complementary role of the three taxa for water quality assessment. Estrogenicity and mutagenicity assays identified with high precision the respective mechanism-specific effects of spikes even when non-specific toxicity occurred in mixture. For estrogenicity, although differences were observed between assays and models, EE2-spike relative induction EC50 values were comparable to the literature, and E2/EE2 equivalency factors reliably reflected the sample content. In the Ames, strong revertant induction occurred following 3-NBA-spike incubation with the TA98 strain, which was of lower magnitude after metabolic transformation and when compared to TA100. Differences in experimental protocols, model organisms, and data analysis can be sources of variation, indicating that respective harmonised standard procedures should be followed when implementing bioassays in water monitoring. Together with other ongoing activities for the validation of a basic bioassay battery, the present study is an important step towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring.
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Di Paolohttps://w3.Ifremer.Fr/archimer-Admin/author.Jsp# Carolina, Ottermanns Richard, Keiter Steffen, Ait-Aissa Selim, Bluhm Kerstin, Brack Werner, Breitholtz Magnus, Buchinger Sebastian, Carere Mario, Chalon Carole, Cousin Xavier, Dulio Valeria, Escher Beate I., Hamers Timo, Hilscherova Klara, Jarque Sergio, Jonas Adam, Maillot-Marechal Emmanuelle, Marneffe Yves, Mai Thao Nguyen, Pandard Pascal, Schifferli Andrea, Schulze Tobias, Seidensticker Sven, Seiler Thomas-Benjamin, Tang Janet, Van Der Oost Ron, Vermeirssen Etienne, Zounkova Radka, Zwart Nick, Hollert Henner (2016). Bioassay battery interlaboratory investigation of emerging contaminants in spiked water extracts – Towards the implementation of bioanalytical monitoring tools in water quality assessment and monitoring. Water Research, 104, 473-484. Publisher's official version : , Open Access version :