FN Archimer Export Format
PT J
TI Distinct immune responses of juvenile and adult oysters (Crassostrea gigas) to viral and bacterial infections
BT 
AF GREEN, Timothy J.
   VERGNES, Agnes
   MONTAGNANI, Caroline
   DE LORGERIL, Julien
AS 1:1,2;2:1;3:1;4:1;
FF 1:;2:PDG-RBE-IHPE;3:PDG-RBE-IHPE;4:PDG-RBE-IHPE;
C1 Univ Montpellier, CNRS, IFREMER, IHPE,UMR 5244,Univ Perpignan Via Domitia, F-34095 Montpellier, France.
   Macquarie Univ, Dept Biol Sci, Sydney, NSW 2109, Australia.
C2 IFREMER, FRANCE
   UNIV MACQUARIE, AUSTRALIA
SI MONTPELLIER
SE PDG-RBE-IHPE
UM IHPE
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-int-hors-europe
IF 2.798
TC 36
UR https://archimer.ifremer.fr/doc/00348/45875/45575.pdf
   https://archimer.ifremer.fr/doc/00348/45875/45576.csv
   https://archimer.ifremer.fr/doc/00348/45875/45577.csv
LA English
DT Article
AB Since 2008, massive mortality events of Pacific oysters (Crassostrea gigas) have been reported worldwide and these disease events are often associated with Ostreid herpesvirus type 1 (OsHV-1). Epidemiological field studies have also reported oyster age and other pathogens of the Vibrio genus are contributing factors to this syndrome. We undertook a controlled laboratory experiment to simultaneously investigate survival and immunological response of juvenile and adult C. gigas at different time-points post-infection with OsHV-1, Vibrio tasmaniensis LGP32 and V. aestuarianus. Our data corroborates epidemiological studies that juveniles are more susceptible to OsHV-1, whereas adults are more susceptible to Vibrio. We measured the expression of 102 immune-genes by high-throughput RT-qPCR, which revealed oysters have different transcriptional responses to OsHV-1 and Vibrio. The transcriptional response in the early stages of OsHV-1 infection involved genes related to apoptosis and the interferon-pathway. Transcriptional response to Vibrio infection involved antimicrobial peptides, heat shock proteins and galectins. Interestingly, oysters in the later stages of OsHV-1 infection had a transcriptional response that resembled an antibacterial response, which is suggestive of the oyster's microbiome causing secondary infections (dysbiosis-driven pathology). This study provides molecular evidence that oysters can mount distinct immune response to viral and bacterial pathogens and these responses differ depending on the age of the host.
PY 2016
PD JUN
SO Veterinary Research
SN 0928-4249
PU Biomed Central Ltd
VL 47
IS 72
UT 000380096700001
BP 1
EP 11
DI 10.1186/s13567-016-0356-7
ID 45875
ER

EF