FN Archimer Export Format PT J TI Thermococcus piezophilus sp. nov., a novel hyperthermophilic and piezophilic archaeon with a broad pressure range for growth, isolated from a deepest hydrothermal vent at the Mid-Cayman Rise BT AF DALMASSO, Cecile OGER, Philippe SELVA, Gwendoline COURTINE, Damien L'HARIDON, Stephane GARLASCHELLI, Alexandre ROUSSEL, Erwan MIYAZAKI, Junichi REVEILLAUD, Julie JEBBAR, Mohamed TAKAI, Ken MAIGNIEN, Lois ALAIN, Karine AS 1:1,2,3;2:4;3:1,2,3;4:1,2,3;5:1,2,3;6:1,2,3;7:1,2,3;8:5;9:1,2,3;10:1,2,3;11:5;12:1,2,3;13:1,2,3; FF 1:;2:;3:;4:;5:;6:;7:PDG-REM-EEP-LMEE;8:;9:PDG-REM-EEP-LMEE;10:;11:;12:;13:; C1 Univ Bretagne Occidentale, IUEM, UMR 6197, LM2E,UEB, Pl Nicolas Copernic, F-29280 Plouzane, France. CNRS, IUEM, LM2E, UMR 6197, F-29280 Plouzane, France. IFREMER, UMR 6197, LM2E, Technopole Pointe Diable, F-29280 Plouzane, France. Univ Lyon, CNRS UMR 5240, INSA Lyon, 11 Ave Jean Capelle, F-69621 Villeurbanne, France. Japan Agcy Marine Earth Sci & Technol JAMSTEC, Dept Subsurface Geobiol Anal & Res D SUGAR, 2-15 Natsushima Cho, Yokosuka, Kanagawa 2370061, Japan. C2 UBO, FRANCE CNRS, FRANCE IFREMER, FRANCE UNIV LYON, FRANCE JAMSTEC, JAPAN SI BREST SE PDG-REM-EEP-LMEE UM BEEP-LM2E IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france copubli-int-hors-europe IF 3.931 TC 29 UR https://archimer.ifremer.fr/doc/00348/45949/45655.pdf LA English DT Article DE ;Thermococcus;Piezophile;Hydrothermal vent;Cayman Trough AB A novel strictly anaerobic, hyperthermophilic archaeon, designated strain CDGST, was isolated from a deep-sea hydrothermal vent in the Cayman Trough at 4964 m water depth. The novel isolate is obligate anaerobe and grows chemoorganoheterotrophically with stimulation of growth by sulphur containing compounds. Its growth is optimal at 75 °C, pH 6.0 and under a pressure of 50 MPa. It possesses the broadest hydrostatic pressure range for growth that has ever been described for a microorganism. Its genomic DNA G + C content is 51.11 mol%. The novel isolate belongs to the genus Thermococcus. Phylogenetic analyses indicated that it is most closely related to Thermococcus barossii DSM17882T based on its 16S rRNA gene sequence, and to ‘Thermococcus onnurineus’ NA1 based on its whole genome sequence. The average nucleotide identity scores with these strains are 77.66% for T. barossii and 84.84% for ‘T. onnurineus’, respectively. Based on the draft whole genome sequence and phenotypic characteristics, strain CDGST is suggested to be separated into a novel species within the genus Thermococcus, with proposed name Thermococcus piezophilus (type strain CDGST = ATCC TSD-33T = UBOCC 3296T). PY 2016 PD OCT SO Systematic And Applied Microbiology SN 0723-2020 PU Elsevier Gmbh, Urban & Fischer Verlag VL 39 IS 7 UT 000386187600002 BP 440 EP 444 DI 10.1016/j.syapm.2016.08.003 ID 45949 ER EF