Valorization of the Macroalgae Sargassum Muticum by Enzymatic Hydrolysis, Interest of Surfactants to Improve the Extraction of Phlorotannins and Polysaccharides

Type Article
Date 2016
Language English
Author(s) Vandanjon Laurent2, 4, Vallet Laurent1, 2, Le Glatin Titouan1, Deleris Paul1, Baron RegisORCID3, Bourseau Patrick2, 5, Dumay Justine1
Affiliation(s) 1 : Université de Nantes, MMS, rue de la Houssinière, Nantes, France
2 : Université de Nantes, GEPEA, Boulevard de l’Université, Saint-Nazaire, France
3 : IFREMER, Laboratoire BiorafHE, Unité Biotechnologie et Ressources Marines, Rue de l’Ile d’Yeu, Nantes, France
4 : Université de Bretagne Sud, LBCM, Rue Saint-Maudé, Lorient, France
5 : Université de Bretagne Sud, IRDL, Rue Saint-Maudé, Lorient, France
Source Journal of Marine Biology and Aquaculture (2381-0750) (Ommega Online Publishers), 2016 , Vol. 2 , N. 1 , P. 1-7
DOI 10.15436/2381-0750.16.014
Keyword(s) Brown macroalgae, Sargassum muticum, Enzymatic hydrolysis, Surfactants, Phlorotannins
Abstract The use of surfactants to improve enzymatic hydrolysis of the macroalgae Sargassum muticum has been investigated. Visible absorption spectroscopy has been used to quantify the solubilization of both polysaccharides and phlorotannins in the hydrolysates.
  After total extraction, results showed that Sargassum muticum contained 2.74% (expressed in percent of the dry weight of the algae) of phlorotannins whose 32 % were in the cell wall. This result shows that it is important to access to the parietal phlorotannins. To reach this objective, we chose the enzymatic approach for destructurating the cell wall of the algae. The use of 5% dry weight (DW - 5% by weight of hydrolyzed algae) of an enzymatic mix containing a commercial beta-glucanase, a commercial protease and an alginate lyase extracted from Pseudomonas alginovora led after 3 hours of hydrolysis to the solubilization of 2.43% DW polysaccharides and 0.52% DW phlorotannins. The use of 0.5% volume of the surfactant Triton® X-100 with 10% DW of the enzymatic mix has allowed to reaching the value of 2.63% DW of solubilized phlorotannins, that is 96% of the total phenolic content.
  The use of non-ionic surfactant, combined to enzymatic hydrolysis, showed an increased efficiency in disrupting cell wall and solubilizing phlorotannins in Sargassum muticum.
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Vandanjon Laurent, Vallet Laurent, Le Glatin Titouan, Deleris Paul, Baron Regis, Bourseau Patrick, Dumay Justine (2016). Valorization of the Macroalgae Sargassum Muticum by Enzymatic Hydrolysis, Interest of Surfactants to Improve the Extraction of Phlorotannins and Polysaccharides. Journal of Marine Biology and Aquaculture, 2(1), 1-7. Publisher's official version : https://doi.org/10.15436/2381-0750.16.014 , Open Access version : https://archimer.ifremer.fr/doc/00350/46105/