FN Archimer Export Format PT J TI Valorization of the Macroalgae Sargassum Muticum by Enzymatic Hydrolysis, Interest of Surfactants to Improve the Extraction of Phlorotannins and Polysaccharides BT AF VANDANJON, Laurent VALLET, Laurent LE GLATIN, Titouan DELERIS, Paul BARON, Regis BOURSEAU, Patrick DUMAY, Justine AS 1:2,4;2:1,2;3:1;4:1;5:3;6:2,5;7:1; FF 1:;2:;3:;4:;5:PDG-RBE-BRM;6:;7:; C1 Université de Nantes, MMS, rue de la Houssinière, Nantes, France Université de Nantes, GEPEA, Boulevard de l’Université, Saint-Nazaire, France IFREMER, Laboratoire BiorafHE, Unité Biotechnologie et Ressources Marines, Rue de l’Ile d’Yeu, Nantes, France Université de Bretagne Sud, LBCM, Rue Saint-Maudé, Lorient, France Université de Bretagne Sud, IRDL, Rue Saint-Maudé, Lorient, France C2 UNIV NANTES, FRANCE UNIV NANTES, FRANCE IFREMER, FRANCE UBS, FRANCE UBS, FRANCE SI NANTES SE PDG-RBE-BRM-BIORAPHE PDG-RBE-BRM TC 0 UR https://archimer.ifremer.fr/doc/00350/46105/46073.pdf LA English DT Article DE ;Brown macroalgae;Sargassum muticum;Enzymatic hydrolysis;Surfactants;Phlorotannins AB The use of surfactants to improve enzymatic hydrolysis of the macroalgae Sargassum muticum has been investigated. Visible absorption spectroscopy has been used to quantify the solubilization of both polysaccharides and phlorotannins in the hydrolysates.   After total extraction, results showed that Sargassum muticum contained 2.74% (expressed in percent of the dry weight of the algae) of phlorotannins whose 32 % were in the cell wall. This result shows that it is important to access to the parietal phlorotannins. To reach this objective, we chose the enzymatic approach for destructurating the cell wall of the algae. The use of 5% dry weight (DW - 5% by weight of hydrolyzed algae) of an enzymatic mix containing a commercial beta-glucanase, a commercial protease and an alginate lyase extracted from Pseudomonas alginovora led after 3 hours of hydrolysis to the solubilization of 2.43% DW polysaccharides and 0.52% DW phlorotannins. The use of 0.5% volume of the surfactant Triton® X-100 with 10% DW of the enzymatic mix has allowed to reaching the value of 2.63% DW of solubilized phlorotannins, that is 96% of the total phenolic content.   The use of non-ionic surfactant, combined to enzymatic hydrolysis, showed an increased efficiency in disrupting cell wall and solubilizing phlorotannins in Sargassum muticum. PY 2016 SO Journal of Marine Biology and Aquaculture SN 2381-0750 PU Ommega Online Publishers VL 2 IS 1 BP 1 EP 7 DI 10.15436/2381-0750.16.014 ID 46105 ER EF