FN Archimer Export Format
PT J
TI Colonization of polystyrene microparticles by Vibrio crassostreae: light and electron microscopic investigation
BT 
AF FOULON, Valentin
   LE ROUX, Frederique
   LAMBERT, Christophe
   HUVET, Arnaud
   SOUDANT, Philippe
   PAUL-PONT, Ika
AS 1:1;2:2,3;3:1;4:4;5:1;6:1;
FF 1:;2:PDG-RBE-PFOM;3:;4:PDG-RBE-PFOM-PI;5:;6:;
C1 Inst Univ Europeen Mer, UMR CNRS UBO IRD Ifremer 6539, Lab Sci Environm Marin LEMAR, Technopole Brest Iroise, Rue Dumont Urville, F-29280 Plouzane, France.
   Ifremer, Unite Physiol Fonct Organismes Marins, ZI Pointe Diable, CS 10070, F-29280 Plouzane, France.
   UPMC Paris 06, Sorbonne Univ, CNRS, UMR 8227,Integrat Biol Marine Models,Stn Biol Ros, CS 90074, F-29688 Roscoff, France.
   Ctr Bretagne, ZI Pointe Diable, UMR UBO CNRS IRD Ifremer 6539, Ifremer,Lab Sci Environm Marin LEMAR, CS 10070, F-29280 Plouzane, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
   UNIV PARIS 06, FRANCE
   IFREMER, FRANCE
SI ROSCOFF
   BREST
SE PDG-RBE-PFOM
   PDG-RBE-PFOM-PI
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 6.198
TC 89
UR https://archimer.ifremer.fr/doc/00351/46210/45908.pdf
LA English
DT Article
AB Microplastics collected at sea harbour a high diversity of microorganisms including some Vibrio genus members, raising questions about the role of microplastics as a novel ecological niche for potentially pathogenic microorganisms. In the present study we investigated the adhesion dynamics of Vibrio crassostreae on polystyrene microparticles (micro-PS) using electronic and fluorescence microscopy techniques. Micro-PS were incubated with bacteria in different media (Zobell culture medium and artificial seawater) with or without natural marine aggregates. The highest percentage of colonised particles (38-100%) was observed in Zobell culture medium, which may be related to nutrient availability for production of pili and exopolysaccharide adhesion structures. A longer bacterial attachment (6 days) was observed on irregular micro-PS compared to smooth particles (<10h) but complete decolonisation of all particles eventually occurred. The presence of natural marine agreggates around micro-PS led to substantial and perennial colonisation featuring monospecific biofilms at the surface of the aggregates. These exploratory results suggest that V. crassostreae may be a secondary coloniser of micro-PS, requiring a multi-species community to form a durable adhesion phenotype. Temporal assessment of microbial colonisation on microplastics at sea using imaging and omics approaches are further indicated to better understand the microplastics colonisation dynamics and species assemblages.
PY 2016
PD OCT
SO Environmental Science & Technology
SN 0013-936X
PU Amer Chemical Soc
VL 50
IS 20
UT 000385907200025
BP 10988
EP 10996
DI 10.1021/acs.est.6b02720
ID 46210
ER

EF