FN Archimer Export Format PT J TI Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth BT AF TRAVERS, Marie-Agnes SOW, Cisse ZIRAH, Severine DEREGNAUCOURT, Christiane CHAOUCH, Soraya QUEIROZ, Rayner M. L. CHARNEAU, Sebastien ALLAIN, Thibault FLORENT, Isabelle GRELLIER, Philippe AS 1:1;2:2;3:2;4:2;5:2;6:3;7:3;8:2,4;9:2;10:2; FF 1:PDG-RBE-SG2M-LGPMM;2:;3:;4:;5:;6:;7:;8:;9:;10:; C1 IFREMER, Lab Genet & Pathol Mollusques Marins, Unite SG2M, La Tremblade, France. Sorbonne Univ, CNRS, Museum Natl Hist Nat, MCAM UMR 7245, Paris, France. Univ Brasilia, Inst Biol, Dept Cell Biol, Brasilia, DF, Brazil. AgroParisTech, INRA, Commensal & Probiot Host Interact Lab, UMR 1319, Jouy En Josas, France. C2 IFREMER, FRANCE MNHN, FRANCE UNIV BRASILIA, BRAZIL INRA, FRANCE SI LA TREMBLADE SE PDG-RBE-SG2M-LGPMM IN WOS Ifremer jusqu'en 2018 DOAJ copubli-france copubli-p187 copubli-int-hors-europe copubli-sud IF 4.076 TC 45 UR https://archimer.ifremer.fr/doc/00351/46211/45906.pdf LA English DT Article DE ;giardiasis;lactobacilli;probiotics;microbiota;bile salts;anti-giardial activity;choloylglycine bile acid hydrolase AB Giardiasis, currently considered a neglected disease, is caused by the intestinal protozoan parasite Giardia duodenalis and is widely spread in human as well as domestic and wild animals. The lack of appropriate medications and the spread of resistant parasite strains urgently call for the development of novel therapeutic strategies. Host microbiota or certain probiotic strains have the capacity to provide some protection against giardiasis. By combining biological and biochemical approaches, we have been able to decipher a molecular mechanism used by the probiotic strain Lactobacillus johnsonii La1 to prevent Giardia growth in vitro. We provide evidence that the supernatant of this strain contains active principle(s) not directly toxic to Giardia but able to convert non-toxic components of bile into components highly toxic to Giardia. By using bile acid profiling, these components were identified as deconjugated bile-salts. A bacterial bile-salt-hydrolase of commercial origin was able to mimic the properties of the supernatant. Mass spectrometric analysis of the bacterial supernatant identified two of the three bile-salt-hydrolases encoded in the genome of this probiotic strain. These observations document a possible mechanism by which L. johnsonii La1, by secreting, or releasing BSH-like activity(ies) in the vicinity of replicating Giardia in an environment where bile is present and abundant, can fight this parasite. This discovery has both fundamental and applied outcomes to fight giardiasis, based on local delivery of deconjugated bile salts, enzyme deconjugation of bile components, or natural or recombinant probiotic strains that secrete or release such deconjugating activities in a compartment where both bile salts and Giardia are present. PY 2016 PD SEP SO Frontiers In Microbiology SN 1664-302X PU Frontiers Media Sa VL 7 UT 000384173400001 DI 10.3389/fmicb.2016.01453 ID 46211 ER EF