Fish Reproduction Is Disrupted upon Lifelong Exposure to Environmental PAHs Fractions Revealing Different Modes of Action

Type Article
Date 2016-12
Language English
Author(s) Vignet Caroline1, Larcher Thibaut2, 3, Davail Blandine4, Joassard Lucette5, Le Menach Karyn4, Guionnet Tiphaine5, Lyphout Laura1, 5, Ledevin Mireille2, 3, Goubeau Manon1, Budzinski Helene4, Begout Marie-LaureORCID5, Cousin XavierORCID1, 6, 7
Affiliation(s) 1 : IFREMER, Ecotoxicol Lab, Pl Gaby Coll, F-17137 Lhoumeau, France.
2 : Oniris, INRA UMR703, APEX, F-44307 Nantes, France.
3 : LUNAM Univ, Oniris, Ecole Natl Vet Agroalimentaire & Alimentat Nante, F-44307 Nantes, France.
4 : Univ Bordeaux 1, Dept Sci & Technol, EPOC, UMR CNRS 5805, F-33405 Talence, France.
5 : IFREMER, Fisheries Lab, Pl Gaby Coll, F-17137 Lhoumeau, France.
6 : INRA LPGP, Campus Beaulieu, F-35042 Rennes, France.
7 : IFREMER, Lab Adaptat & Adaptabilite Anim & Syst, UMR MARBEC, Route Maguelone, F-34250 Palavas Les Flots, France.
Source Toxics (2305-6304) (Mdpi Ag), 2016-12 , Vol. 4 , N. 4 , P. 26 (1-18)
DOI 10.3390/toxics4040026
WOS© Times Cited 7
Note This article belongs to the Special Issue Zebrafish as a Model for Assessing Chemical Toxicity
Keyword(s) polycyclic aromatic hydrocarbons, zebrafish, spawning success, fertilization, gonad differentiation, molecular mechanisms
Abstract Polycyclic aromatic hydrocarbons (PAHs) constitute a large family of organic pollutants emitted in the environment as complex mixtures, the compositions of which depend on origin. Among a wide range of physiological defects, PAHs are suspected to be involved in disruption of reproduction. In an aquatic environment, the trophic route is an important source of chronic exposure to PAHs. Here, we performed trophic exposure of zebrafish to three fractions of different origin, one pyrolytic and two petrogenic. Produced diets contained PAHs at environmental concentrations. Reproductive traits were analyzed at individual, tissue and molecular levels. Reproductive success and cumulative eggs number were disrupted after exposure to all three fractions, albeit to various extents depending on the fraction and concentrations. Histological analyses revealed ovary maturation defects after exposure to all three fractions as well as degeneration after exposure to a pyrolytic fraction. In testis, hypoplasia was observed after exposure to petrogenic fractions. Genes expression analysis in gonads has allowed us to establish common pathways such as endocrine disruption or differentiation/maturation defects. Taken altogether, these results indicate that PAHs can indeed disrupt fish reproduction and that different fractions trigger different pathways resulting in different effects.
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Vignet Caroline, Larcher Thibaut, Davail Blandine, Joassard Lucette, Le Menach Karyn, Guionnet Tiphaine, Lyphout Laura, Ledevin Mireille, Goubeau Manon, Budzinski Helene, Begout Marie-Laure, Cousin Xavier (2016). Fish Reproduction Is Disrupted upon Lifelong Exposure to Environmental PAHs Fractions Revealing Different Modes of Action. Toxics, 4(4), 26 (1-18). Publisher's official version : https://doi.org/10.3390/toxics4040026 , Open Access version : https://archimer.ifremer.fr/doc/00355/46641/