FN Archimer Export Format
PT J
TI Pyrococcus kukulkanii sp nov., a hyperthermophilic, piezophilic archaeon isolated from a deep-sea hydrothermal vent
BT 
AF CALLAC, Nolwenn
   OGER, Philippe
   LESONGEUR, Francoise
   RATTRAY, Jayne E.
   VANNIER, Pauline
   MICHOUD, Gregoire
   BEAUVERGER, Mickael
   GAYET, Nicolas
   ROUXEL, Olivier
   JEBBAR, Mohamed
   GODFROY, Anne
AS 1:1,2,3,4,5;2:6;3:1,2,3;4:5;5:1,2,3;6:1,2,3;7:1,2,3;8:7;9:8;10:1,2,3;11:1,2,3;
FF 1:;2:;3:PDG-REM-EEP-LMEE;4:;5:;6:;7:;8:PDG-REM-EEP-LEP;9:PDG-REM-GM-LCG;10:;11:PDG-REM-EEP-LMEE;
C1 Univ Brest, Lab Microbiol Environm Extremes, UEB, IUEM,UMR 6197, 4 Rue Dumont Urville, F-29280 Plouzane, France.
   IFREMER, Lab Microbiol Environm Extremes, UMR 6197, Technopole Brest Iroise,CS10070, F-29280 Plouzane, France.
   CNRS, Lab Microbiol Environm Extremes, UMR 6197, 4 Rue Dumont Urville, F-29280 Plouzane, France.
   Univ Brest, Domaines Ocean IUEM, UMR 6538, 4 Rue Dumont Urville, F-29280 Plouzane, France.
   Stockholm Univ, Dept Geol Sci, Stockholm, Sweden.
   Univ Lyon, INSA Lyon, CNRS, UMR 5240, Lyon, France.
   IFREMER, Lab Environm Profonds, Technopole Brest Iroise,CS10070, F-29280 Plouzane, France.
   IFREMER, Lab Geochim & Metallogenie, Technopole Brest Iroise,CS10070, F-29280 Plouzane, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
   CNRS, FRANCE
   UBO, FRANCE
   UNIV STOCKHOLM, SWEDEN
   UNIV LYON, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
   PDG-REM-EEP-LEP
   PDG-REM-GM-LCG
UM BEEP-LM2E
   LGO
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 2.134
TC 12
UR https://archimer.ifremer.fr/doc/00356/46682/46540.pdf
LA English
DT Article
CR BIG
BO L'Atalante
DE ;Hyperthermophile;Archaea;Pyrococcus;Piezophile;Thermococcales
AB A novel hyperthermophilic, piezophilic, anaerobic archaeon, designated NCB100(T), was isolated from a hydrothermal vent flange fragment collected in the Guaymas basin at the hydrothermal vent site named 'Rebecca's Roost' at a depth of 1997 m. Enrichment and isolation were performed at 100 degrees C under atmospheric pressure. Cells of strain NCB100(T) were highly motile, irregular cocci with a diameter of -1 mu m. Growth was recorded at temperatures between 70 and 112 degrees C (optimum 105 degrees C) and hydrostatic pressures of 0.1-80 MPa (optimum 40-50 MPa). Growth was observed at pH 3.5-8.5 (optimum pH 7) and with 1.5-7% NaCl (optimum at 2.5-3 %). Strain NCB100(T) was a strictly anaerobic chemo-organoheterotroph and grew on complex proteinaceous substrates such as yeast extract, peptone and tryptone, as well as on glycogen and starch. Elemental sulfur was required for growth and was reduced to hydrogen sulfide. The fermentation products from complex proteinaceous substrates were CO2 and H-2. The G+C content of the genomic DNA was 41.3 %. Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain NCB100(T) belongs to the genus Pyrococcus, showing 99% similarity with the other described species of the genus Pyrococcus. On the basis of physiological characteristics, DNA G+C content, similarity level between ribosomal proteins and an average nucleotide identity value of 79 %, strain NCB100(T) represents a novel species for which the name Pyrococcus kukulkanii sp. nov. is proposed. The type strain is NCB100(T) (=DSM 101590(T) =Souchotheque de Bretagne BG1337(T)).
PY 2016
PD AUG
SO International Journal Of Systematic And Evolutionary Microbiology
SN 1466-5026
PU Microbiology Soc
VL 66
UT 000384920900055
BP 3142
EP 3149
DI 10.1099/ijsem.0.001160
ID 46682
ER

EF