FN Archimer Export Format
PT J
TI Morphological and genetic diversity of Beaufort Sea diatoms with high contributions from the     Chaetoceros neogracilis     species complex
BT 
AF BALZANO, Sergio
   PERCOPO, Isabella
   SIANO, Raffaele
   GOURVIL, Priscillia
   CHANOINE, Melanie
   MARIE, Dominique
   VAULOT, Daniel
   SARNO, Diana
AS 1:1,4;2:2;3:3;4:1;5:1;6:1;7:1;8:2;
FF 1:;2:;3:PDG-ODE-DYNECO-PELAGOS;4:;5:;6:;7:;8:;
C1 UPMC Univ Paris 06, Sorbonne Univ, CNRS, Stn Biol Roscoff,UMR 7144, F-29680 Roscoff, France.
   Stn Zool Anton Dohrn, Integrat Marine Ecol Dept, I-80121 Naples, Italy.
   IFREMER, Dyneco Pelagos, BP 70, F-29280 Plouzane, France.
   Nioz Royal Netherlands Inst Sea Res, Dept Marine Microbiol & Biogeochem, POB 59, NL-1790 AB Texel, Netherlands.
C2 UNIV PARIS 06, FRANCE
   STN ZOOL ANTON DOHRN, ITALY
   IFREMER, FRANCE
   INST SEA RESEARCH (NIOZ), NETHERLANDS
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 3
TC 45
UR https://archimer.ifremer.fr/doc/00356/46718/46584.pdf
LA English
DT Article
DE ;biogeography;ITS;ITS2 secondary structure;LSU;morphology;phylogeny;polar diatoms;SSU
AB Seventy-five diatoms strains isolated from the Beaufort Sea (Canadian Arctic) in the summer of 2009 were characterized by light and electron microscopy (SEM and TEM) as well as 18S and 28S rRNA gene sequencing. These strains group into 20 genotypes and 17 morphotypes and are affiliated with the genera Arcocellulus, Attheya, Chaetoceros, Cylindrotheca, Eucampia, Nitzschia, Porosira, Pseudo-nitzschia, Shionodiscus, Thalassiosira, Synedropsis. Most of the species have a distribution confined to the northern/polar area. Chaetoceros neogracilis and Chaetoceros gelidus were the most represented taxa. Strains of C. neogracilis were morphologically similar and shared identical 18S rRNA gene sequences, but belonged to four distinct genetic clades based on 28S rRNA, ITS-1 and ITS-2 phylogenies. Secondary structure prediction revealed that these four clades differ in hemi-compensatory base changes (HCBCs) in paired positions of the ITS-2, suggesting their inability to interbreed. Reproductively isolated C. neogracilis genotypes can thus co-occur in summer phytoplankton communities in the Beaufort Sea. Chaetoceros neogracilis generally occurred as single cells but can also form short colonies. It is phylogenetically distinct from an Antarctic species, erroneously identified in some previous studies as C. neogracilis but named here as Chaetoceros sp. This work provides taxonomically validated sequences for 20 Arctic diatom taxa, which will facilitate future metabarcoding studies on phytoplankton in this region.
PY 2017
PD FEB
SO Journal Of Phycology
SN 0022-3646
PU Wiley
VL 53
IS 1
UT 000397259800015
BP 161
EP 187
DI 10.1111/jpy.12489
ID 46718
ER

EF