FN Archimer Export Format
PT J
TI The highly variable microbiota associated to intestinal mucosa correlates with growth and hypoxia resistance of sea bass, Dicentrarchus labrax, submitted to different nutritional histories
BT 
AF GATESOUPE, Francois-Joel
   HUELVAN, Christine
   LE BAYON, Nicolas
   LE DELLIOU, Herve
   MADEC, Lauriane
   MOUCHEL, Olivier
   QUAZUGUEL, Patrick
   MAZURAIS, David
   ZAMBONINO-INFANTE, Jose-Luis
AS 1:1,3;2:2;3:2;4:2;5:2;6:2;7:2;8:2;9:2;
FF 1:;2:PDG-RBE-PFOM-ARN;3:PDG-RBE-PFOM-ARN;4:PDG-RBE-PFOM-ARN;5:PDG-RBE-PFOM-ARN;6:PDG-RBE-PFOM-ARN;7:PDG-RBE-PFOM-ARN;8:PDG-RBE-PFOM-ARN;9:PDG-RBE-PFOM-ARN;
C1 Univ Pau & Pays Adour, INRA, NUMEA, F-64310 St Pee Sur Nivelle, France.
   IFREMER, PFOM ARN, Ctr Bretagne, UMR LEMAR 6539, CS 10070, F-29280 Plouzane, France.
   IFREMER, PFOM ARN, Ctr Bretagne, CS 10070, F-29280 Plouzane, France.
C2 INRA, FRANCE
   IFREMER, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-RBE-PFOM-ARN
UM LEMAR
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-p187
IF 2.644
TC 46
UR https://archimer.ifremer.fr/doc/00356/46719/46590.pdf
   https://archimer.ifremer.fr/doc/00356/46719/46592.pptx
   https://archimer.ifremer.fr/doc/00356/46719/46593.pptx
   https://archimer.ifremer.fr/doc/00356/46719/46594.pptx
   https://archimer.ifremer.fr/doc/00356/46719/46595.pptx
   https://archimer.ifremer.fr/doc/00356/46719/46596.pdf
   https://archimer.ifremer.fr/doc/00356/46719/46597.pdf
   https://archimer.ifremer.fr/doc/00356/46719/46598.pdf
   https://archimer.ifremer.fr/doc/00356/46719/46599.pdf
   https://archimer.ifremer.fr/doc/00356/46719/46600.pdf
   https://archimer.ifremer.fr/doc/00356/46719/46601.pdf
   https://archimer.ifremer.fr/doc/00356/46719/46602.pptx
   https://archimer.ifremer.fr/doc/00356/46719/46603.pdf
LA English
DT Article
DE ;Host-microbe interaction;16S rRNA;Pyrosequencing;Autochthonous bacteria;Alternative feed ingredients;Physiological status
AB Background  The better understanding of how intestinal microbiota interacts with fish health is one of the key to sustainable aquaculture development. The present experiment aimed at correlating active microbiota associated to intestinal mucosa with Specific Growth Rate (SGR) and Hypoxia Resistance Time (HRT) in European sea bass individuals submitted to different nutritional histories: the fish were fed either standard or unbalanced diets at first feeding, and then mixed before repeating the dietary challenge in a common garden approach at the juvenile stage. Results  A diet deficient in essential fatty acids (LH) lowered both SGR and HRT in sea bass, especially when the deficiency was already applied at first feeding. A protein-deficient diet with high starch supply (HG) reduced SGR to a lesser extent than LH, but it did not affect HRT. In overall average, 94 % of pyrosequencing reads corresponded to Proteobacteria, and the differences in Operational Taxonomy Units (OTUs) composition were mildly significant between experimental groups, mainly due to high individual variability. The highest and the lowest Bray-Curtis indices of intra-group similarity were observed in the two groups fed standard starter diet, and then mixed before the final dietary challenge with fish already exposed to the nutritional deficiency at first feeding (0.60 and 0.42 with diets HG and LH, respectively). Most noticeably, the median percentage of Escherichia-Shigella OTU_1 was less in the group LH with standard starter diet. Disregarding the nutritional history of each individual, strong correlation appeared between (1) OTU richness and SGR, and (2) dominance index and HRT. The two physiological traits correlated also with the relative abundance of distinct OTUs (positive correlations: Pseudomonas sp. OTU_3 and Herbaspirillum sp. OTU_10 with SGR, Paracoccus sp. OTU_4 and Vibrio sp. OTU_7 with HRT; negative correlation: Rhizobium sp. OTU_9 with HRT). Conclusions  In sea bass, gut microbiota characteristics and physiological traits of individuals are linked together, interfering with nutritional history, and resulting in high variability among individual microbiota. Many samples and tank replicates seem necessary to further investigate the effect of experimental treatments on gut microbiota composition, and to test the hypothesis whether microbiotypes may be delineated in fish.
PY 2016
PD NOV
SO Bmc Microbiology
SN 1471-2180
PU Biomed Central Ltd
VL 16
IS 266
UT 000387083300006
BP 1
EP 13
DI 10.1186/s12866-016-0885-2
ID 46719
ER

EF