FN Archimer Export Format PT J TI A semi-automated Raman micro-spectroscopy method for morphological and chemical characterizations of microplastic litter BT AF FRERE, L. PAUL-PONT, I. MOREAU, Julien SOUDANT, P. LAMBERT, C. HUVET, Arnaud RINNERT, Emmanuel AS 1:1;2:1;3:2;4:1;5:1;6:3;7:2; FF 1:;2:;3:PDG-REM-GM-LES;4:;5:;6:PDG-RBE-PFOM-PI;7:PDG-REM-RDT-LDCM; C1 Inst Univ Europeen Mer, Lab Sci Environm Marin LEMAR, UMR 6539, CNRS UBO IRD Ifremer, F-29280 Plouzane, France. IFREMER, Lab Detect Capteurs & Mesures, CS 10070, F-29280 Plouzane, France. CNRS UBO IRD Ifremer, IFREMER, LEMAR UMR 6539, CS 10070, F-29280 Plouzane, France. C2 UBO, FRANCE IFREMER, FRANCE IFREMER, FRANCE SI BREST SE PDG-REM-GM-LES PDG-RBE-PFOM-PI PDG-REM-RDT-LDCM UM LEMAR IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 3.146 TC 119 UR https://archimer.ifremer.fr/doc/00357/46802/46976.pdf LA English DT Article DE ;Microplastics;Raman micro-spectroscopy;Surface seawater;Morphology;Environmental monitoring;Automating AB Every step of microplastic analysis (collection, extraction and characterization) is time-consuming, representing an obstacle to the implementation of large scale monitoring. This study proposes a semi-automated Raman micro-spectroscopy method coupled to static image analysis that allows the screening of a large quantity of microplastic in a time-effective way with minimal machine operator intervention. The method was validated using 103 particles collected at the sea surface spiked with 7 standard plastics: morphological and chemical characterization of particles was performed in < 3 h. The method was then applied to a larger environmental sample (n = 962 particles). The identification rate was 75% and significantly decreased as a function of particle size. Microplastics represented 71% of the identified particles and significant size differences were observed: polystyrene was mainly found in the 2–5 mm range (59%), polyethylene in the 1–2 mm range (40%) and polypropylene in the 0.335–1 mm range (42%). PY 2016 PD DEC SO Marine Pollution Bulletin SN 0025-326X PU Pergamon-elsevier Science Ltd VL 113 IS 1-2 UT 000390734600075 BP 461 EP 468 DI 10.1016/j.marpolbul.2016.10.051 ID 46802 ER EF