Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France

Type Article
Date 2016-12
Language English
Author(s) Polo David1, Schaeffer Julien, Fournet Nelly2, Le Saux Jean-Claude1, Parnaudeau Sylvain1, McLeod Catherine3, Le Guyader Soizick1
Affiliation(s) 1 : IFREMER, Nantes, France.
2 : French Natl Publ Hlth Agcy, Sante Publ France, St Maurice, France.
3 : Seafood Safety Assessment Ltd, Killmore, Isle Of Skye, Scotland.
Source Emerging Infectious Diseases (1080-6040) (Centers Disease Control), 2016-12 , Vol. 22 , N. 12 , P. 2189-2191
DOI 10.3201/eid2212.160841
WOS© Times Cited 14
Abstract Using samples from oysters clearly implicated in human disease, we quantified norovirus levels by using digital PCR. Concentrations varied from 43 to 1,170 RNA copies/oyster. The analysis of frozen samples from the production area showed the presence of norovirus 2 weeks before consumption.
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Polo David, Schaeffer Julien, Fournet Nelly, Le Saux Jean-Claude, Parnaudeau Sylvain, McLeod Catherine, Le Guyader Soizick (2016). Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France. Emerging Infectious Diseases, 22(12), 2189-2191. Publisher's official version : https://doi.org/10.3201/eid2212.160841 , Open Access version : https://archimer.ifremer.fr/doc/00359/46971/