Pseudodesulfovibrio indicus gen. nov., sp nov., a piezophilic sulfate-reducing bacterium from the Indian Ocean and reclassification of four species of the genus Desulfovibrio

Type Article
Date 2016-10
Language English
Author(s) Cao Junwei1, 2, 3, 4, 5, 6, 7, 8, Gayet Nicolas9, Zeng Xiang5, 6, 7, 8, Shao Zongze5, 6, 7, 8, Jebbar Mohamed1, 2, 3, Alain Karine1, 2, 3
Affiliation(s) 1 : UBO, UEB, IUEM, UMR 6197,LMEE, Pl Nicolas Copernic, F-29280 Plouzane, France.
2 : CNRS, IUEM, UMR 6197, LMEE, Pl Nicolas Copernic, F-29280 Plouzane, France.
3 : IFREMER, UMR 6197, LMEE, Technopole Pointe Diable, F-29280 Plouzane, France.
4 : Harbin Inst Technol, Sch Municipal & Environm Engn, Harbin 150090, Peoples R China.
5 : State Key Lab Breeding Base Marine Genet Resource, Xiamen, Peoples R China.
6 : Third Inst State Ocean Adm, Key Lab Marine Genet Resources, Xiamen, Peoples R China.
7 : Collaborat Innovat Ctr Marine Biol Resources, Xiamen, Peoples R China.
8 : Key Lab Marine Genet Resources Fujian Prov, Xiamen, Peoples R China.
9 : IFREMER, Ctr Brest, REM, EEP,LEP,Inst Carnot,EDROME, F-29280 Plouzane, France.
Source International Journal Of Systematic And Evolutionary Microbiology (1466-5026) (Microbiology Soc), 2016-10 , Vol. 66 , P. 3904-3911
DOI 10.1099/ijsem.0.001286
WOS© Times Cited 14
Abstract A novel sulfate-reducing bacterium, strain J2(T), was isolated from a serpentinized peridotite sample from the Indian Ocean. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J2(T) clustered with the genus Desulfovibrio within the family Desulfovibrionaceae, but it showed low similarity (87.95 %) to the type species Desulfovibrio desulfuricans DSM 642(T). It was most closely related to Desulfovibrio portus MSL79(T) (96.96 %), followed by Desulfovibrio aespoeensis Aspo-2(T) (96.11 %), Desulfovibrio piezophilus C1TLV30(T) (96.04 %) and Desulfovibrio profundus DSM 11384(T) (95.17 %). Other available sequences shared less than 93.33 % 16S rRNA gene sequence similarity. Cells were Gram-staining-negative, anaerobic, motile vibrios (2-6 x 0.4-0.6 mu m). Growth was observed at salinities ranging from 0.2 to 6 % (optimum 2.5 %), from pH 5 to 8 (optimum pH 6.5-7) and at temperatures between 9 and 40 degrees C (optimum 30-35 degrees C). J2(T) was piezophilic, growing optimally at 10 MPa (range 0-30 MPa). J2(T) used lactate, malate, pyruvate, formate and hydrogen as energy sources. Sulfate, thiosulfate, sulfite, fumarate and nitrate were used as terminal electron acceptors. Lactate and pyruvate were fermented. The main fatty acids were iso-C-15:0, anteiso-C-15:0, summed feature 9 (iso-C-17:1 omega 9c and/or C-16:0 10-methyl) and iso-C-17:0. The DNA G+C content of strain J2(T) was 63.5 mol%. The combined genotypic and phenotypic data show that strain J2(T) represents a novel species of a novel genus in the family Desulfovibrionaceae, for which the name Pseudodesulfovibrio indicus gen. nov., sp. nov. is proposed, with the type strain J2(T) (= MCCC 1A01867(T) = DSM 101483(T)). We also propose the reclassification of D. piezophilus as Pseudodesulfovibrio piezophilus comb. nov., D. profundus as Pseudodesulfovibrio profundus comb. nov., D. portus as Pseudodesulfovibrio portus comb. nov. and D. aespoeensis as Pseudodesulfovibrio aespoeensis comb. nov.
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Cao Junwei, Gayet Nicolas, Zeng Xiang, Shao Zongze, Jebbar Mohamed, Alain Karine (2016). Pseudodesulfovibrio indicus gen. nov., sp nov., a piezophilic sulfate-reducing bacterium from the Indian Ocean and reclassification of four species of the genus Desulfovibrio. International Journal Of Systematic And Evolutionary Microbiology, 66, 3904-3911. Publisher's official version : https://doi.org/10.1099/ijsem.0.001286 , Open Access version : https://archimer.ifremer.fr/doc/00359/47018/